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PC138 Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Nuclear Factor ΚB

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
      Catalogue NumberPC138
      Brand Family Calbiochem®
      SynonymsAnti-Nuclear Factor ΚB
      References
      ReferencesKolberg, R., 1997. J. NIH Research 9, 29.
      Van Antwerp, D.J. et al. 1996. Science 274, 787.
      Beg, A.A., et al. 1995. Nature 376, 167.
      Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
      Wulczyn, F.G., et al. 1992. Nature 358, 597.
      Product Information
      FormLiquid
      FormulationIn 5 mM sodium phosphate buffer, pH 7.4.
      Positive controlDaudi cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (see comments)
      Gel Shift (see comments)
      Immunoblotting (1:500, chemiluminescence)
      Immunoprecipitation (see comments)
      Application CommentsThis antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PC138 0

      Documentation

      Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      PC138

      Literatur

      Übersicht
      Kolberg, R., 1997. J. NIH Research 9, 29.
      Van Antwerp, D.J. et al. 1996. Science 274, 787.
      Beg, A.A., et al. 1995. Nature 376, 167.
      Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
      Wulczyn, F.G., et al. 1992. Nature 358, 597.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-October-2007 RFH
      SynonymsAnti-Nuclear Factor ΚB
      ApplicationELISA (see comments)
      Gel Shift (see comments)
      Immunoblotting (1:500, chemiluminescence)
      Immunoprecipitation (see comments)
      DescriptionRabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
      BackgroundNFκB (Nuclear factor κB) is a transcription factor that plays crucial roles in gene regulation including those involved in immune and inflammatory responses. Active NFκB is most commonly composed of the heterodimer DNA binding subunits p50 and p65 (RelA). These subunits are members of a multigene family called the rel family. In addition to p50 and RelA, three other proteins are implicated in dimer formation leading to transcriptional activation, p52, c-rel and rel B. Inhibitors to this process exist including another member of the rel family IκBα which binds to c-rel, p65 and relB preventing DNA binding by their homo- or heterodimers. It has recently been shown that NFκB plays a role in the apoptotic process - when RelA, the gene that codes for an NFκB subunit, was knocked out, this lead to the death of mouse embryos through apoptosis of the liver cells. Similarly, it has been shown in a wide range of cells that NFκB which has been inactivated by IκBα were more sensitive to TNF-α-induced apoptosis than control cells. Evidence exists for NFκB playing both anti- and pro-apoptotic roles - it has been suggested that different combinations of subunits may result in different outcomes in terms of cell survival.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
      IsotypeIgG
      Specieshuman
      Positive controlDaudi cells
      FormLiquid
      FormulationIn 5 mM sodium phosphate buffer, pH 7.4.
      Preservative≤0.1% sodium azide
      CommentsThis antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKolberg, R., 1997. J. NIH Research 9, 29.
      Van Antwerp, D.J. et al. 1996. Science 274, 787.
      Beg, A.A., et al. 1995. Nature 376, 167.
      Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
      Wulczyn, F.G., et al. 1992. Nature 358, 597.