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MABS1905 Anti-TMEPAI Antibody, clone 9F10

This mouse monoclonal Anti-TMEPAI, Antibody, clone 9F10, Cat. No. MABS1905 is used in Immunocytochemistry, Immunoprecipitation, and Western blotting for the detection of TMEPAI.

Anti-TMEPAI Antibody, clone 9F10: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: PMEPA1, STAG1, TMEPAI UniProt Number: Q969W9
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MABS1905
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1905
      DescriptionAnti-TMEPAI Antibody, clone 9F10
      Alternate Names
      • Prostate transmembrane protein androgen induced 1
      • Solid tumor-associated 1 protein
      • Transmembrane prostate androgen-induced protein
      Background InformationProtein TMEPAI (UniProt: Q969W9; also known as Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein) is encoded by the PMEPA1 (also known as STAG1, TMEPAI) gene (Gene ID: 56937) in human. TMEPAI is a type Ib transmembrane protein containing two TMEPAI contains 2 WW-binding motifs and a SMAD interaction motif. The WW-binding motifs mediate its interaction with NEDD4 and the SMAD interaction motif is required for interaction with SMAD2 and SMAD3 and for the negative regulation of TGF-beta signaling. TMEPAI antagonizes TGF-beta signaling by interfering with TGF-beta type I receptor-induced R-SMAD phosphorylation. It competes with SMAD anchor for receptor activation for R-SMAD binding, thereby sequestering R-SMADs from TGF-beta receptor I kinase activation. By acting as a negative regulator of TGF-beta signaling, TMEPAI plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production, and immunosuppression. Higher expression of TMEPAI is observed in prostate and ovaries and the expression can be up-regulated by androgen and TGF-beta (at protein level).TMEPAI is constitutively and highly expressed in many types of cancer and has been implicated in poor prognosis. TMEPAI is also reported to be involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR. (Ref.: Nguyen THV et al. (2014). Cancer Sci. 105: 334 341; Watanabe, Y et al. (2010). Mol. Cell 37(1): 123-134).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-TMEPAI, Antibody, clone 9F10, Cat. No. MABS1905 is used in Immunocytochemistry, Immunoprecipitation, and Western blotting for the detection of TMEPAI.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot detected TMEPAI in Immunoprecipitation applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).

      Western Blotting Analysis: A representative lot detected TMEPAI in lysate from COS-7 cells overexpressing TEMPAI. (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan).

      Western Blotting Analysis: 8 ug/mL from a representative lot detected TMEPAI in TGF-beta stimulated HaCaT cell lysates (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan).

      Immunocytochemistry Analysis: A representative lot detected TMEPAI in Immunocytochemistry applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).

      Western Blotting Analysis: A representative lot detected TMEPAI in Western Blotting applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 39 amino acids from the C-terminal region of human TMEPAI.
      Clone9F10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9F10 detects TMEPAI in COS-7 cells overexpressing TEMPAI. It targets an epitope of 39 amino acids from the C-terminal region in isoform 1. Also shows reactivity with isoform 2 that has 100% sequence homology in this epitope region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman, Monkey.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PMEPA1
      • STAG1
      • TMEPAI
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~32 kDa observed; 31.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in COS-7 overexpressing TMEPAI lysate.

      Western Blotting Analysis: 0.02 µg/mL of this antibody detected TMEPAI in 45 µg of lysate from COS-7 cells overexpressing TMEPAI.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1905 04054839176456