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MABF3107-100UG Anti-ULBP2 Antibody, clone BUMO1

Anti-ULBP2, clone BUMO1, Cat. No. MABF3107, is a mouse monoclonal antibody that detects ULBP2 and is tested for use in ELISA and Flow Cytometry.

MABF3107-100UG
100 μg  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberMABF3107-100UG
      DescriptionAnti-ULBP2 Antibody, clone BUMO1
      Alternate Names
      • UL16-binding protein 2
      • ALCAN-alpha
      • NKG2D ligand 2
      • N2DL-2
      • NKG2DL2
      • Retinoic acid early transcript 1H
      Background InformationUL16-binding protein 2 (UniProt: Q9BZM5; also known as ALCAN-alpha, NKG2D ligand 2, N2DL-2, NKG2DL2, Retinoic acid early transcript 1H) is encoded by the ULBP2 (also known as N2DL2, RAET1H, UNQ463/PRO791) gene (Gene ID: 80328) in human. The UL16-binding proteins (ULBPs) belong to the family of MHC class I-related molecules that are targets of human CMV glycoprotein. ULBP2 is a GPI-anchor glycoprotein that is synthesized with a signal peptide (aa 1-25) and a propeptide (aa 217-246), which are subsequently cleaved off to produce the mature form. ULBP2 serves as a ligand of the activating immunoreceptor NKG2D and mediates natural kill (NK) cell cytotoxicity. It is detected in cell membrane and endoplasmic reticulum. A secreted for has also been described. Its expression is observed in cancer cells and in the fetus, but not in normal tissues. Tumor cells are reported to release the soluble form of ULBP2 by the action of metalloproteases, however, unlike membrane bound form, it does not down-regulate NKG2D on NK cells. Soluble form of ULBP2 is frequently detected in sera of patients with hematopoietic malignancies. NKG2D, frequently expressed on tumor cells, stimulate tumor immunity. Soluble forms of ULBPs are also reported to induce protein tyrosine phosphorylation, activation of Janus kinase 2, MAP kinase, and PI-3 kinase/Akt signaling. Clone BUMO-1 specifically detects ULBP2 and is suitable for multiple immunodetection applications. (Ref.: Waldhauer, I., and Steinle, A. (2006). Cancer Res 66(5); 2520-2526; Welte, SA., et al. (2003). Eur. J. Immunol. 33(1); 194-203; Sutherland, CL., et al. (2002). J. Immunol. 168(2); 671-679).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ULBP2, clone BUMO1, Cat. No. MABF3107, is a mouse monoclonal antibody that detects ULBP2 and is tested for use in ELISA and Flow Cytometry.
      Key Applications
      • Flow Cytometry
      • ELISA
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected ULBP2 in ELISA applications (Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Weil, S., et al. (2017). Front Immunol. 10:8:387).

      Flow Cytometry Analysis: A representative lot detected ULBP2 in Flow Cytometry applications (Welte, S.A., et al. (2003). Eur J Immunol. 33(1):194-203; Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Heinemann, A., et al. (2012). Cancer Res. 72(2):460-71).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      Immunogenp815 murine mastocytoma cells expressing human ULBP2.
      EpitopeUnknown
      CloneBUMO1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone BUMO1 is a mouse monoclonal antibody that detects UL16-binding protein 2 (ULBP2).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ULBP2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight27.37 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in C1R cells expressing ULBP2.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected ULBP2 in one million C1R cells expressing ULBP2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF3107-100UG 04065271059085