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69041 B834(DE3) Competent Cells - Novagen

B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.

B834(DE3) Competent Cells - Novagen: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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      Übersicht

      Replacement Information
      Description
      OverviewB834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).

      DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.



      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Genotype: F- ompT hsdSB(rB- mB-) gal dcm met (DE3)


      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69041
      Brand Family Novagen®
      References
      References1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

      2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
      Product Information
      Components
      Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/μg
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      69041 0

      Documentation

      B834(DE3) Competent Cells - Novagen SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      B834(DE3) Competent Cells - Novagen Analysenzertifikate

      TitelChargennummer
      69041

      Literatur

      Übersicht
      1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.

      2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.

      Broschüre

      Titel
      Competent Cell Brochure

      Literaturstellen

      Titel
    • Rossi, F., et al. 2008. Journal of Biological Chemistry 283, 3559.
    • Xue, X., et al. 2008. Journal of Virology 82, 2515.
    • Buetow, L., et al. 2007. Procedings of the National Academy of Science 104, 4321.
    • Alderwick, L.J., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 2558.
    • Chédin, F., et al. 2006. Journal of Biological Chemistry 281, 18610.
    • Sasaki, H.M., et al. 2006. Procedings of the National Academy of Science 103, 14744.
    • Sheckler, L.R., et al. 2006. Journal of Biological Chemistry 281, 22896.
    • Song, F., et al. 2006. Journal of Biological Chemistry 281, 11028.
    • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
    • Dey, S., et al. 2005. Journal of Biological Chemistry 280, 14892.
    • Maria- Eugenia Guazzaroni, M-F., et al. 2005. Journal of Biological Chemistry 280, 20887.
    • Jogl, G., et al. 2005. i>Journal of Biological Chemistry 280, 738.
    • Li, H., et al. 2005. Protein Science 14, 3025.
    • Li, K., et al. 2005. Journal of Biological Chemistry 280, 29176.
    • Singh, S., et al. 2005. Journal of Biological Chemistry 280, 17101.
    • Zhu, G., et al. 2005. Journal of Biological Chemistry 280, 23057.
    • Chen, Y-h., et al. 2004. Nature. 429, 675.
    • Hsieh, T-J., et al. 2004. Journal of Biological Chemistry 279, 55587.
    • Lehmann, C., et al. 2004. Proteins: Structure, Function, and Bioinformatics 56, 376.
    • Mancini, E.J., et al. 2004. Acta Crystallographica D60, 588.
    • Nguyen, H., et al. 2004. Journal of Structural and Functional Genomics 5, 23.
    • James S. Parker, S. Mark Roe and David Barford. 2004. European Molecular Biology Organization Journal 23, 4727.
    • Welin, M., et al. 2004. Procedings of the National Academy of Science 101, 17970.
    • Obmolova, G., et al. 2000. Nature 407, 703.
    • David K. Worthylake, Kent L. Rossman and John Sondek. 2000. Nature 408, 682.
    • Conn, G.L., et al. 1999. Science 284, 1171.
    • Joey M. Studts and Brian G, Fox. 1999. Protein Expression and Purification 16, 109.
    • Cabral, J.H.M., et al. 1998. Cell 95, 649.
    • Fauman, E.B., et al. 1998. Cell 93, 617.
    • Gomis-Ruth, F.-X., et al. 1997. Nature 389, 77.
    • Dyda, F., et al. 1994. Science 266, 1981.
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      Anwendungsvorschriften

      Titel
      TB009 Competent Cells
      TB053 Academic and Non-profit Laboratory Assurance Letter

      Verwandte Produkte & Anwendungen

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      Bestellnummer Beschreibung  
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      Kategorien

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression