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FC010-100MG Sigma-AldrichHuman Plasma Fibronectin Purified Protein

Human Plasma Fibronectin Purified Protein: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

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Key Applications: Cell Culture

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FC010-100MG

100 mg

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Übersicht

Replacement Information

Key Spec Table

Key Applications
CULT

Description
Catalogue Number	FC010-100MG
Replaces	FC010-250MG
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Human Plasma Fibronectin Purified Protein
Overview	Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

References

Product Information
Presentation	Lyophilized from 50 mM tris-buffered saline (TBS), pH 7.5, containing no preservatives.
Quality Level	MQ100

Applications
Key Applications	Cell Culture
Application Notes	Immunochemical standard Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts Suggested Procedure for Coating Cell Cultureware 1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2. 2. Reconstitute the entire vial* of hFN to 1mg/mL a sterile balanced salt solution at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1). 3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area. 4. Introduce the proper CO2 atmosphere, if required. 5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below. 6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes. 7. Remove residual balanced salt solution before proceeding with standard cell culture procedures. *Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.

Applications

Key Applications

Cell Culture

Application Notes

Immunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2.

2. Reconstitute the entire vial* of hFN to 1mg/mL a sterile balanced salt solution at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1).

3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

4. Introduce the proper CO2 atmosphere, if required.

5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below.

6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes.

7. Remove residual balanced salt solution before proceeding with standard cell culture procedures.

*Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.

Biological Information
Purity	Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
Source	Human donor plasma.
Entrez Gene Number	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
Entrez Gene Summary	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProt Number	P02751
UniProt Summary	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Stem Cell Type	Human Embryonic Stem Cells Mesenchymal Stem Cells Neural Stem Cells Hematopoietic Stem Cells Epithelial Cells Pancreatic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months from date of receipt. Store the reconstituted material at 2º-8° C for up to 6 months.

Packaging Information
Material Size	100 mg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Bestellnummer	GTIN
FC010-100MG	04053252474866

Documentation

Human Plasma Fibronectin Purified Protein SDB

Titel
Sicherheitsdatenblatt (SDB)

Human Plasma Fibronectin Purified Protein Analysenzertifikate

Titel	Chargennummer
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2390364	2390364
HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2024988	2024988
HUMAN PLASMA FIBRONECTIN - 3170851	3170851
HUMAN PLASMA FIBRONECTIN - 3209787	3209787
HUMAN PLASMA FIBRONECTIN - 3231559	3231559
HUMAN PLASMA FIBRONECTIN - 3317808	3317808
HUMAN PLASMA FIBRONECTIN - 3352012	3352012
HUMAN PLASMA FIBRONECTIN - 3429096	3429096
HUMAN PLASMA FIBRONECTIN - 3436730	3436730
HUMAN PLASMA FIBRONECTIN - 3727566	3727566

Literatur

Übersicht	Pub Med ID
Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system. Seltana, Amira, et al. Wound Repair Regen, 18: 114-22 2009 Abstract anzeigen Rapid and efficient healing of epithelial damage is critical to the functional integrity of the small intestine. Epithelial repair is a complex process that has largely been studied in cultured epithelium but to a much lesser extent in mucosa. We describe a novel method for the study of wound healing using a co-culture system that combined an intestinal epithelial Caco-2/15 cell monolayer cultured on top of human intestinal myofibroblasts, which together formed a basement membrane-like structure that contained many of the major components found at the epithelial-mesenchymal interface in the human intestine. To investigate the mechanism of restitution, small lesions were generated in epithelial cell monolayers on plastic or in co-cultures without disturbing the underlying mesenchymal layer. Monitoring of wound healing showed that repair was more efficient in Caco-2/15-myofibroblast co-cultures than in Caco-2/15 monolayers and involved the deposition of basement membrane components. Functional experiments showed that the addition of type I collagen or human fibronectin to the culture medium significantly accelerated wound closure on epithelial cell co-cultures. This system may provide a new tool to investigate the mechanisms that regulate wound healing in the intestinal epithelium.	20082684
Molecular properties of wild-type and mutant betaIG-H3 proteins Kim, Jung-Eun, et al Invest Ophthalmol Vis Sci, 43:656-661 (2002) 2002	11867580
Comparative studies on amniotic fluid and plasma fibronectins Ruoslahti E. et al. Biochem. J, 193:295-299 (1981) 1981	7305927

Broschüre

Titel
Human Stem Cell Systems

Datenblatt

Titel
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

Produktfamilien

Kategorien

Life Science Research > Proteins and Enzymes > ECM Proteins for Plate Coating

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FC010-100MG Sigma-AldrichHuman Plasma Fibronectin Purified Protein

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