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613548 TMB, Insoluble

TMB, Insoluble: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: 3,3ʹ,5,5ʹ-Tetramethylbenzidine

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      Descripción

      Replacement Information
      Description
      OverviewPeroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.
      Catalogue Number613548
      Brand Family Calbiochem®
      Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
      References
      Product Information
      CAS number54827-17-7
      FormClear to light brown liquid
      Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSDV2300000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      613548 0

      Documentation

      TMB, Insoluble Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      TMB, Insoluble Certificados de análisis

      CargoNúmero de lote
      613548
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2008 RFH
      Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
      DescriptionInsoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H2O2), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H2O2, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes
      FormClear to light brown liquid
      Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
      Recommended reaction conditionsSuggested Procedure for Immunoblot Staining with TMB: 1. Complete all required incubations with antibodies and HRP-labeled probes. 2. Wash membranes thoroughly with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween®-20 detergent. Note: Do not use reagents containing thimerosal or phenolic compounds as preservatives. 3. Following the final wash, cover the membranes completely with TMB solution and incubate with gentle rocking for 5-30 min, or until the desired color intensity is obtained (aquamarine/blue bands or dots will appear at the sites of enzyme activity). Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops immediately, the TMB precipitate may flake off the membrane. If this occurs, further dilution of the HRP probe is recommended. A fine blue line circumscribing the band or dot also suggests that dilution of the enzyme is necessary. 4. Stop the reaction by washing the membrane thoroughly in distilled water. DO NOT WASH WITH BUFFERED SOLUTIONS OR TAP WATER. These will cause fading. Also, DO NOT USE ACIDS TO STOP THE REACTION. Acids will turn the bands yellow. Excessive background staining indicates incomplete removal of unbound HRP from membranes. To remedy this problem, increase the wash steps or washing times. 5. Air-dry membranes and store protected from light.
      CAS number54827-17-7
      RTECSDV2300000
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
      Toxicity Carcinogenic / Teratogenic