AB5320A4 | Anti-NG2, Alexa Fluor®488 Conjugate Antibody

AB5320A4
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, H, MkICC, IHCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue NumberAB5320A4
      DescriptionAnti-NG2, Alexa Fluor®488 Conjugate Antibody
      Alternate Names
      • Chondroitin sulfate proteoglycan 4
      • Chondroitin sulfate proteoglycan NG2
      • Melanoma chondroitin sulfate proteoglycan
      • Melanoma-associated chondroitin sulfate proteoglycan
      Background InformationNG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Rat oligodendrocyte precursor cells
      PresentationPurified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationAnti-NG2, Alexa Fluor®488 Conjugate Antibody detects level of NG2 & has been published & validated for use in ICC & IHC.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in adult rat brain tissue.
      Biological Information
      ImmunogenImmunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat
      HostRabbit
      SpecificityThis antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      • Monkey
      Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Mouse, Human, and Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MCSP
      Purification MethodProtein A adsorption
      UniProt Number
      UniProt SummaryFUNCTION: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

      SIZE: 2322 amino acids; 250497 Da

      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.

      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).

      SIMILARITY: Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.

      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells
      Molecular WeightThe unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NG2, Alexa Fluor#174;488 Conjugate - 2031361 2031361
      Anti-NG2, Alexa Fluor#174;488 Conjugate - 2201520 2201520
      Anti-NG2, Alexa Fluor#174;488 Conjugate - 2262709 2262709
      Anti-NG2, Alexa Fluor#174;488 Conjugate - Q1988592 Q1988592
      Anti-NG2, Alexa Fluor®488 Conjugate 2449241
      Anti-NG2, Alexa Fluor®488 Conjugate 2897249
      Anti-NG2, Alexa Fluor®488 Conjugate 2869686
      Anti-NG2, Alexa Fluor®488 Conjugate - 2391193 2391193
      Anti-NG2, Alexa Fluor®488 Conjugate - 2343141 2343141
      Anti-NG2, Alexa Fluor®488 Conjugate - 2512046 2512046

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies