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ST1027 Anti-Acetylated Lysine Rabbit pAb

Anti-Acetylated Lysine, rabbit polyclonal antibody that recognizes acetylated lysine in UV-treated HEK293 cells. It is validated for ELISA, Western blotting, immunoprecipitation, & paraffin sections.

Anti-Acetylated Lysine Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1027
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesRbPolyclonal Antibody

Pricing & Availability

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ST1027-50UL
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      		 Plastic ampoule 50 ul
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      Description
      OverviewRecognizes acetylated lysine residues in UV-treated HEK293 cells.
      Catalogue NumberST1027
      Brand Family Calbiochem®
      Application Data
      Detection of monkey acetylated lysine by immunoblotting. Samples: Cell extracts from COS cells, untreated or TSA-treated (lanes 1 and 2) and serum starved (lanes 3 and 4). Primary antibody: Anti-Acetylated Lysine Rabbit pAb (Cat. No. ST1027) (1:1000). Detection: chemiluminescence.
      References
      ReferencesYu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
      Mal, A., et al. 2001. EMBO J. 20, 1739.
      Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlHEK293 cells treated with UV
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesELISA (1:1000)
      Immunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Paraffin Sections (1:50)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogensynthetic acetylated-lysine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1027-50UL 04055977208672

      Documentation

      Anti-Acetylated Lysine Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Acetylated Lysine Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1027

      References

      Reference overview
      Yu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
      Mal, A., et al. 2001. EMBO J. 20, 1739.
      Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.

      Brochure

      Title
      Antibody Sourcebookﾙ Sixth Edition
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-October-2007 RFH
      ApplicationELISA (1:1000)
      Immunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Paraffin Sections (1:50)
      Application Data
      Detection of monkey acetylated lysine by immunoblotting. Samples: Cell extracts from COS cells, untreated or TSA-treated (lanes 1 and 2) and serum starved (lanes 3 and 4). Primary antibody: Anti-Acetylated Lysine Rabbit pAb (Cat. No. ST1027) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes acetylated lysine-containing proteins.
      BackgroundProtein acetylation is a reversible modification that can control the activity of histones, p53, and many other proteins. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs).
      HostRabbit
      Immunogensynthetic acetylated-lysine
      IsotypeIgG
      Speciesa broad range of species
      Positive controlHEK293 cells treated with UV
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesYu, X., et al. 2002. J. Natl. Cancer Inst. 94, 504.
      Mal, A., et al. 2001. EMBO J. 20, 1739.
      Sano, Y. and Ishii, S. 2001. J. Biol. Chem. 276, 3674.