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MAB1087-I-25UG Anti-Eosinophil Peroxidase Antibody, clone AHE-1

Anti-Eosinophil Peroxidase, clone AHE-1, Cat. No. MAB1087-I, is a mouse monoclonal antibody that detects Eosinophil Peroxidase and is tested for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry.

MAB1087-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1087-I-25UG
      DescriptionAnti-Eosinophil Peroxidase Antibody, clone AHE-1
      Alternate Names
      • EPO
      • EPX
      Background InformationEosinophil peroxidase (UniProt: P11678; also known as EC:1.11.1.7, EPO, EPX) is encoded by the EPX (also known as EPER, EPO, EPP) gene (Gene ID: 8288) in human. Eosinophil peroxidase is an enzyme found in cytoplasmic granules of eosinophils. It mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. It is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-139), which are subsequently cleaved off to produce the mature form that can be cleaved into a light chain (aa 140-250) and a heavy chain (aa 251-715). These chains can assemble to for a tetrameric structure. It is a heme-containing enzyme that physically associates with fibrous tissue and cancer tissue in various organs. It contains well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional extracellular matrix (ECM) both in vitro and in vivo. It plays a role in regulating the recruitment of fibroblast and the biosynthesis of collagen ECM at sites of normal tissue repair and fibrosis. It is a key participant in generation of reactive oxidants and diffusible radical species by the phagocytes. It serves as a potent toxin towards invading parasites and the surrounding tissues. It also displays significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. (Ref.: DeNichilo, MO., et al. (2015). Am. J. Pathol. 185(5); 1372-1384; Mitra, SN., et al. (2000). Redox Rep. 5(4); 215-224).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Eosinophil Peroxidase, clone AHE-1, Cat. No. MAB1087-I, is a mouse monoclonal antibody that detects Eosinophil Peroxidase and is tested for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesTested applications

      Immunofluorescence Analysis: A representative lot detected Eosinophil Peroxidase in Immunofluorescence applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821).

      Immunohistochemistry Applications: A representative lot detected Eosinophil Peroxidase in Immunohistochemistry applications (Spang, C., et al. (2017). J Musculoskelet Neuronal Interact. 17(3); 226-236; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821; Song, Y., et al. (2013). BMC Musculoskelet Disord. 14:; 34; Song, Y., et al. (2012). PLoS One ;7(12); e52230).

      Immunocytochemistry Analysis: A representative lot detected Eosinophil Peroxidase in Immunocytochemistry applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHuman Eosinophils from a patient with hypereosinophilic syndrome.
      EpitopeUnknown
      CloneAHE-1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AHE-1 is a mouse monoclonal antibody that detects Eosinophil Peroxidase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EPX
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight81.04 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunofluorescence in frozen Human colon tissue sections.

      Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Eosinophil Peroxidase in frozen Human colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1087-I-25UG 04065269802358