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ST1630 Anti-FGF8 Mouse mAb (2A10)

Anti-FGF8, mouse monoclonal, clone 2A10, recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. It is validated for use in ELISA and Western blotting.

Anti-FGF8 Mouse mAb (2A10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fibroblast Growth Factor 8

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ST1630
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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ST1630-100UG
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      Description
      OverviewRecognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells.
      Catalogue NumberST1630
      Brand Family Calbiochem®
      SynonymsAnti-Fibroblast Growth Factor 8
      Application Data
      Detection of rat FGF8 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse FGF8 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlPC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein
      Clone2A10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1630-100UG 04055977223736

      Documentation

      Anti-FGF8 Mouse mAb (2A10) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-Fibroblast Growth Factor 8
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of rat FGF8 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse FGF8 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein.
      BackgroundThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.
      HostMouse
      ImmunogenFull-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein
      Clone2A10
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlPC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review