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ABE2696 Anti-GADD45 alpha Antibody

This Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.

Anti-GADD45 alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: GADD45A, DDIT1, GADD45 UniProt Number: P24522
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ABE2696
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2696
      Replaces07-1230
      DescriptionAnti-GADD45 alpha Antibody
      Alternate Names
      • Growth arrest and DNA damage-inducible protein GADD45 alpha
      • DDIT-1
      • DNA damage-inducible transcript 1 protein
      Background InformationGrowth arrest and DNA damage-inducible protein GADD45 alpha (UniProt P24522; also known as DDIT-1, DNA damage-inducible transcript 1 protein) is encoded by the GADD45A (also known as DDIT1, GADD45) gene (Gene ID 1647) in human. GADD45A, GADD45B, and GADD45G constitute a family of sensors that can mediate rapid responses to a variety of stressors in mammals. GADD45 proteins are involved in cellular DNA repair, cell cycle, cell survival, apoptosis, senescence, and DNA demethylation under various stress conditions. The interaction of GADD45 with PCNA regulates DNA replication and repair by stimulating nucleotide excision repair. GADD45 can induce cell cycle arrest through direct interaction with cell cycle-related protein CDK1 and p21. Direct phosphorylation of MEKK4 by GADD45 leads to activation of JNK and p38 kinase, resulting in cellular apoptosis and senescence, respectively. All GADD45 proteins are able to interact with each other and form homo- or hetero-dimers, which are crucial for GADD45 functions. GADD45 proteins are ubiquitously expressed, but can vary in their tissue expression patterns among the three members. Gadd45a deletion in mice causes genomic instability, resulting in defective mitosis and cytokinesis, as wll as increased radiation carcinogenesis. In addition to the reported antitumor activity via the induction of cellular apoptosis or senescence in cancer cells, GADD45A has also been shown to play a role in facilitating tumorigenesis in certain cancers.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected GADD45 alpha dimer in 10 µg of HEK293 cell lysate.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GADD45 alpha in human colon cancer tissue section.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal sequence of human GADD45 alpha.
      EpitopeN-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects GADD45 alpha. Immunogen sequence is not conserved in GADD45 beta.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GADD45A
      • DDIT1
      • GADD45
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~18/36 kDa (monomer/dimer) observed. 18.34 kDa (monomer) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD45 alpha in 10 µg of mouse kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2696 04055977355369