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MABT1495-25UG Anti-PD2 (PAF1) Antibody, clone F169-3B2

Anti-PD2 (PAF1), clone F169-3B2, Cat. No. MABT1495, is mouse monoclonal antibody that detects Pancreatic differentiation protein 2 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-PD2 (PAF1) Antibody, clone F169-3B2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PAF1, PD2 UniProt Number: Q8N7H5
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MABT1495-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1495-25UG
      DescriptionAnti-PD2 (PAF1) Antibody, clone F169-3B2
      Alternate Names
      • Pancreatic differentiation protein 2
      • RNA polymerase II-associated factor 1 homolog
      • hPAF1
      Background InformationPancreatic differentiation protein 2 (UniProt: Q8N7H5; also known as RNA polymerase II-associated factor 1 homolog, PD2, hPAF1) is encoded by the PAF1 (also known as PD2) gene (Gene ID: 54623) in human. PD2 is a nuclear protein that serves as a subunit of the RNA polymerase II associated factor (PAF) complex. It has multiple functions during transcription by RNA polymerase II. It has been implicated in regulation of development and maintenance of embryonic stem cell pluripotency. It associates with RNA polymerase II through interaction with POLR2A C-terminal domain non-phosphorylated and serine 2 and serine 5 phosphorylated forms. It is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. Three isoforms of PD2 have been described that are produced by alternative splicing. PD2 has oncogenic property and is shown to be overexpressed in pancreatic cancer cells. In normal murine pancreas, its expression is restricted to acinar cells, however, its expression is increased in the ductal cells of mouse model of pancreatic cancer with advancing age. (Ref.: Dey, P., et al (2014). Oncotarget, 5(12); 4480-4491).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-PD2 (PAF1), clone F169-3B2, Cat. No. MABT1495, is mouse monoclonal antibody that detects Pancreatic differentiation protein 2 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).

      ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).

      Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91).

      Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2).
      CloneF169-3B2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PAF1
      • PD2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight59.98 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Panc1 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PD2 (PAF1) in Panc1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1495-25UG 04054839557880