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MABN2629-25UG Anti-Poly-Neu5Ac Antibody, clone 12E3

Anti-Poly-Neu5Ac, clone 12E3, Cat. No. MABN2629, is a mouse monoclonal antibody that detects Poly-Neu5Ac and is tested for use in ELISA, Flow Cytometry, Immunoprecipitation, and Western Blotting.

MABN2629-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2629-25UG
      DescriptionAnti-Poly-Neu5Ac Antibody, clone 12E3
      Alternate Names
      • N-Acetylneuraminic acid
      • NANA
      Background InformationCell surface of vertebrate cells contains a dense layer of glycans that often terminate with sialic acids, mainly Neu5Ac or Neu5Gc that differ only by a single oxygen atom. Glycoproteins and proteoglycans containing these moieties provide a scaffold for cell-to-cell and cell to environment communication. These sialic acids are mainly present as monosialyl residues at the non-reducing terminus of glycoproteins and play a role in ligand-receptor interactions during fertilization, development, differentiation, and adult brain functions. However, occasionally, sialic acids are linked to each other to form a polymerized structure (poly-sia; poly-Neu5Ac) that can contain 8 to 200 sialyl residues. These glycans are not only modifications of core molecules that are helpful as markers on particular cells, but also have independent functions via their interactions with glycan-recognizing molecules, such as lectins. Neu5Ac is reported to be a prevalent on a variety of sialoglycoconjugates SGCs) in vertebrate brain tissue. Its homopolymeric form (Poly-Neu5Ac) is shown to be most prevalent on the neural cell adhesion molecule (NCAM) and in the capsules of certain neuroinvasive bacteria. NCAM with polymeric Neu5Ac is highly expressed during the embryonic and early postnatal development and plays a critical role in neurite outgrowth and neural plasticity. Poly-Neu5Ac also acts as a neurotropic factor in neuroinvasive E. coli K1 and Neisseria meningitidis serogroup B. The exact mechanisms for breakdown of polymeric forms is not well understood. However, it can undergo an intramolecular self-cleavage into short oligomers under prolonged mild acidic conditions found in lysosomes. Clone 12E3 is highly specific for detection of poly-Neu5Ac glycotype and do not display reactivity towards poly-Neu5Gc. Naito-Matsui, Y., et al. (2017). J. Biol. Chem. 292(7); 2557-2570; Davies, LRL., et al. (2012). J. Biol. Chem. 287(34); 28917-28931; Sato, C., et al. (1995). J. Biol. Chem. 270(32); 18923-18928).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Poly-Neu5Ac, clone 12E3, Cat. No. MABN2629, is a mouse monoclonal antibody that detects Poly-Neu5Ac and is tested for use in ELISA, Flow Cytometry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      • ELISA
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Poly-Neu5Ac in Flow Cytometry applications (Davies, L.R.L., et al. (2012). J Biol Chem. 287(34):28917-31).

      Western Blotting Analysis: A representative lot detected Neu5Ac in Western Blotting applications (Yabe, U., et al. (2003). J Biol Chem. 278(16):13875-80; Naito-Matsui, Y., et al. (2017). J Biol Chem. 292(7):2557-2570).

      Immunoprecipitation Analysis: A representative lot detected Neu5Ac in Immunoprecipitation applications (Yabe, U., et al. (2003). J Biol Chem. 278(16):13875-80).

      ELISA Analysis: A representative lot detected Neu5Ac in ELISA applications (Sato, C., et al. (1995). J Biol Chem. 270(32):18923-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenSplenocytes from mouse injected with forebrain region of E18 rat embryo.
      EpitopeUnknown
      Clone12E3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 12E3 is a mouse monoclonal antibody that detects poly-N-Acetylneuraminic acid (Neu5Ac).
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodLigatrap
      UniProt Number
      Molecular Weight~260 kDa observed in one day old mouse brain tissue extract. 309.27 Da for monomeric Neu5Ac.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in one day old Mouse brain tissue extract.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Poly-Neu5Ac in one day old Mouse brain tissue extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2629-25UG 04065270321664