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MABT403 Anti-Proteinase 3/PR3 Antibody, clone MCPR3-7

Anti-Proteinase 3/PR3 Antibody, clone MCPR3-7 is an antibody against PR3 for use in Affinity Binding Assay, Flow Cytometry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: PRTN3, CANCA, MBN, NP4 UniProt Number: P24158
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MABT403
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ABA, FC, IP, INHIBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT403
      DescriptionAnti-Proteinase 3/PR3 Antibody, clone MCPR3-7
      Alternate Names
      • Myeloblastin
      • AGP7
      • C-ANCA antigen
      • Leukocyte proteinase 3
      • Neutrophil proteinase 4
      • NP-4
      • P29
      • PR-3
      • PR3
      • Wegener autoantigen
      Background InformationMyeloblastin (EC 3.4.21.76; UniProt P24158; also known as AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, NP-4, P29, PR-3, PR3, Wegener autoantigen) is encoded by the PRTN3 (also known as CANCA, MBN, NP4) gene (Gene ID 5657) in human. Proteinase 3 (PR3) is one of four neutral serine proteases (elastase, cathepsin G, PR3, and neutrophil serine protease 4) stored as fully processed mature enzymes in azurophil granules of human neutrophils. Instead of being targeted to granules after their synthesis, some PR3 molecules end up on the surface of neutrophil plasma membrane in either pro- or mature form. The degree of such surface expression is genetically determined, but the surface exposure and pericellular activity of PR3 around neutrophils can be further upregulated upon neutrophil priming and activation. PR3 autoantibody (anti-neutrophil cytoplasmic antibodies or ANCA) is the main pathogenic feature in patients suffering from granulomatosis with polyangiitis (GPA; formerly called Wegener granulomatosis). ANCAs are shown to activate cytokine-primed neutrophils in vitro by cross-linking surface-exposed PR3 and Fcγ receptors. PR3 activity and/or its inactivation by alpha 1-antitrypsin/alpha-1 proteinase inhibitor (α1PI) varies in the human population and contributes to the risk for GPA manifestations either at onset, during relapses, or during systemic progression. PR3 is initially produced with a signal peptide sequence (a.a. 1-25), an N-terminal and a C-terminal propeptide sequence (a.a. 26-27 and 249-256, respectively), the removal of which yields the mature enzyme (a.a. 28-248).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Proteinase 3/PR3 Antibody, clone MCPR3-7 is an antibody against PR3 for use in Affinity Binding Assay, Flow Cytometry, Immunoprecipitation, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Affinity Binding Assay
      • Flow Cytometry
      • Immunoprecipitation
      • Inhibits Activity/Function
      Application NotesWestern Blotting Analysis: 1-2 µg/mL from a representative lot detected 1 µg of recombinant human pro-PR3 under non-reducing condition, but not under reducing condition (Courtesy of Amber Hummel, Mayo Clinic, Rochester, MN).
      Flow Cytometry Analysis: A representative lot immunostained the surface of only a small subpopulation of human peripheral blood neutrophils that are stained positive with clone MCPR3-2 (Cat. No. MABT340), consistent with the surface PR3 being in an active conformation complexed with CD177 and/or lipid bilayers. (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      Flow Cytometry Analysis: A representative lot bound recombinant human PR3-, but not murine PR3-, coated Talon-beads as determined by FACS analysis. (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
      Flow Cytometry Analysis: A representative lot bound immobilized recombinant human PR3 via a distinct epitope than those recognized by clone MCPR3-2 and MCPR3-3 (Cat. No. MABT340 and MABF973, respectively) as determined by FACS analysis of bead-based competition binding assay (Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated a recombinant human PR3 construct proP-PR3ctp that adopts a pro-PR3 conformation, while exhibiting greater than 75% reduced affinity toward a recombinant construct ΔPR3ctp-S195A that adopts a mature PR3 conformation (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      Inhibition Analysis: A representative lot caused a substrate-dependent inhibition against human PR3 enzymatic activity by altering PR3 S1' pocket conformation, but not PR3 S1 pocket conformation (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      Affinity Binding Assay: A representative lot captured a recombinant human PR3 construct proP-PR3ctp that adopts a pro-PR3 conformation, while exhibiting greater than 75% reduced affinity toward a recombinant construct ΔPR3ctp-S195A that adopts a mature PR3 conformation (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      Biological Information
      ImmunogenRecombinant proP-PR3ctp (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      EpitopeA hydrophobic region of the activation domain (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648; Silva, F., et al. (2010). J. Autoimmun. 35(4) 299-308).
      CloneMCPR3-7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone MCPR3-7 targets a hydrophobic region of the activation domain involved in the binding of human PR3 to neutrophil membrane receptor NB1/CD177. Clone MCPR3-7 preferentially recognizes PR3 in a pro-form conformation and causes a substrate-dependent inhibition against human PR3 enzymatic activity by altering PR3 S1' pocket conformation, but not PR3 S1 pocket conformation (Hinkofer, L.C., et al. (2013). J. Biol. Chem. 288(37):26635-26648).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRTN3
      • CANCA
      • MBN
      • NP4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~32 kDa observed. 27.81 kDa (Pro-PR3; a.a. 1-256), 25.14 kDa (N-terminal signal and propeptide removed; a.a. 28-256), 24.25 kDa (Mature; a.a. 28-248) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG1κ.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT403 04055977333619

      Documentation

      Anti-Proteinase 3/PR3 Antibody, clone MCPR3-7 Certificates of Analysis

      TitleLot Number
      Anti-Proteinase 3/PR3, -Q2667551 Q2667551
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 3184985 3184985
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 3234454 3234454
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 3328862 3328862
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 3880956 3880956
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 4107720 4107720
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 4190476 4190476
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 4194243 4194243
      Anti-Proteinase 3/PR3, clone MCPR3-7 - 4275524 4275524

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies