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ABE103 Anti-SIRT7 Antibody

Use Anti-SIRT7 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect SIRT7 also known as NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7.

Anti-SIRT7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: SIRT7, SIR2L7 UniProt Number: Q9NRC8
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ABE103
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE103
      DescriptionAnti-SIRT7 Antibody
      Alternate Names
      • NAD-dependent deacetylase sirtuin-7
      • SIR2-like protein 7
      Background InformationThe silent information regulator (SIR) genes (sirtuins) are a highly conserved family of proteins, which contain one or more sirtuins present in various species from bacteria to mammals. There are seven sirtuin genes that encode seven sirtuin enzymes, SIRT1 through SIRT7, all of which are dependent on oxidized nicotinamide adenine dinucleotide (NAD+). These proteins serve as mono-ADP-ribosyltransferases and deacetylate histones as a means of silencing specific chromatin regions. Further research is still required to elicidate the link between its effects on cellular metabolism, stress resistance, and cellular proliferation and its proposed role in mediating general lifespan. It is reported that Sirt7 expression demonstrates an anti-proliferative role with tumorigenic potential, in several murine cell lines.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Human liver tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-SIRT7 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect SIRT7 also known as NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:00 dilution from a representative lot detected the expressed protein of SIRT7 in human liver tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of the expressed protein of human SIRT7.
      EpitopeN-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of the expressed protein of SIRT7.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
      Gene Symbol
      • SIRT7
      • SIR2L7
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.

      CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

      COFACTOR: Binds 1 zinc ion per subunit.

      SUBUNIT LOCATION: Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus > nucleolus. Note: Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

      Molecular Weight~45 kDa observed. An additional isoform at ~20 kDa is known to exist. An uncharacterized band at ~30 kDa has been observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human liver tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected the expressed protein of SIRT7 in 10 µg of human liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE103 04053252420849