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MABE354 Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1

Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1, Cat. No. MABE354, is a highly specific mouse monoclonal antibody, that targets UNG and has been tested in Western Blotting and Immunohistochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: UNG, DGU, UNG1, UNG15 UniProt Number: P13051
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MABE354
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE354
      DescriptionAnti-Uracil-DNA glycosylase Antibody, clone 8G10.1
      Alternate Names
      • UDG
      • Uracil-DNA glycosylase
      Background InformationUracil-DNA glycosylase (EC 3.2.2.27; UniProt P13051; also known as UDG) is encoded by the UNG (also known as DGU, HIGM4, HIGM5, UNG1, UNG2, UNG15) gene (Gene ID 7374) in human. Uracil-DNA glycosylases catalyze the release of uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway that plays an important role in mutagenesis prevention. Uracil bases in DNA can result from cytosine deamination or misincorporation of dUMP instead of dTTP during DNA synthesis. Alternative promoter usage and splicing of UNG gene lead to two different isoforms, the mitochondrial UNG1 and the nuclear UNG2. The mammalian shelterin complex contains the six proteins (TRF1, TRF2, RAP1, TIN2, POT1, and TPP1), the complex occupies the end of the telomeres and protect telomeres against inappropriate DNA repair when telomeres are not being lengthened. UNG deficiency in primary mouse hematopoietic cells is shown to affect POT1 (Protector of Telomeres 1) recognition of the telomere repeats due to increased uracil incorporation in telomeres TTAGGG repeats, which in turn leads to abnormal telomere lengthening.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Uracil-DNA glycosylase Antibody, clone 8G10.1, Cat. No. MABE354, is a highly specific mouse monoclonal antibody, that targets UNG and has been tested in Western Blotting and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Uracil-DNA glycosylase in human skeletal muscle and human cardiac tissues.
      Biological Information
      ImmunogenLinear peptide corresponding to human Uracil-DNA glycosylase near the C-terminus.
      EpitopeNear C-terminus
      Clone8G10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms (UNG1 & UNG2) of human Uracil-DNA glycosylase.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey, Chimpanzee based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UNG
      • DGU
      • UNG1
      • UNG15
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~34 kDa observed (33.9 kDa/UNG1 & 34.6 kDa/UNG2 calculated). Immunogen sequence should allow to detect both isoform 1 and 2. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Uracil-DNA glycosylase in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE354 04055977176902

      Documentation

      Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1 Certificates of Analysis

      TitleLot Number
      Anti-Uracil-DNA glycosylase, -Q2583339 Q2583339
      Anti-Uracil-DNA glycosylase, clone 8G10.1 - 3587089 3587089

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies