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MABE1906-25UL Anti-Venus (YFP) Antibody, clone 5

Anti-Venus (YFP), clone 5, Cat. No. MABE1906, is a mouse monoclonal antibody that detects proteins labeled with mVenus and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MABE1906-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1906-25UL
      DescriptionAnti-Venus (YFP) Antibody, clone 5
      Alternate Names
      • GFP(YFP)
      • GFP(F46L)
      Background InformationGreen fluorescent protein (UniProt: P42212; also known as GFP) is encoded by the GFP gene in Aequorea victoria (Jelly Fish). GFP is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP has evolved into an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications. Venus is a genetic mutant with F46L substitution in green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies. Compared to other fluorescent tags, Venus provides higher expression levels and better yields in preparative purifications. A fusion protein consisting of Venus and a defective mutant of p27 protein (p27K ) has been used to identify and isolate a population of quiescent cells and visualize the G0 to G1 transition. Clone 5 specifically detects only the Venus-tagged proteins. (Ref.: Nagai, T., et al. (2002). Nat. Biotechnol. 20(1); 87-90; Oki, T., et al. (2014). Sci. Rep. 4; Article 4012; Rana, MS., et al. (2018). Biochemistry 57(49); 6741-6751).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Venus (YFP), clone 5, Cat. No. MABE1906, is a mouse monoclonal antibody that detects proteins labeled with mVenus and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Venus (YFP) in NIH3T3 (L20V-9-11) cells (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

      Western Blotting Analysis: A 1:250 dilution from a representative lot detected Venus (YFP) in HEK293T cells transfected with mVenus that was expressed using pCAGGS-Myc eukaryotic expression plasmid (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

      Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Venus (YFP) labeled proteins in pCAGGS-myc (negative) and pCAGGS-myc-mVenus (positive) HEK293T cell lysates (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).
      Biological Information
      ImmunogenGST‐tagged full-length mVenus, a genetic mutant of GFP derived from Aequorea Victoria (Jelly fish).
      EpitopeUnknown
      Clone5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 5 is a mouse monoclonal antibody that detects proteins labeled with Venus (YFP). It does not display cross-reactivity with mCherry, mRuby, or mClover.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAequorea victoria. Predicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 26.88 kDa calculated for GFP. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1906-25UL 04065265237772

      Documentation

      Anti-Venus (YFP) Antibody, clone 5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Venus (YFP) Antibody, clone 5 Certificates of Analysis

      TitleLot Number
      Anti-Venus (YFP), clone 5 - 3953610 3953610
      Anti-Venus (YFP), clone 5 - 4036279 4036279
      Anti-Venus (YFP), clone 5 - 4037104 4037104
      Anti-Venus (YFP), clone 5 - 4042600 4042600
      Anti-Venus (YFP), clone 5 - 4045893 4045893
      Anti-Venus (YFP), clone 5 - 4054816 4054816
      Anti-Venus (YFP), clone 5 - 4063591 4063591
      Anti-Venus (YFP), clone 5 - 4107738 4107738
      Anti-Venus (YFP), clone 5 - 4122556 4122556
      Anti-Venus (YFP), clone 5 - 4149942 4149942
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