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ABC80 Anti-phospho AMBRA1 Antibody (Ser52)

Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.

Anti-phospho AMBRA1 Antibody (Ser52) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: AMBRA1 UniProt Number: Q9C0C7
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ABC80
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC80
      DescriptionAnti-phospho AMBRA1 Antibody (Ser52)
      Alternate Names
      • Activating molecule in BECN1-regulated autophagy protein 1
      • Ambra
      • Ambra-1
      Background InformationActivating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesWestern Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA.

      Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52.
      EpitopePhosphorylated Ser52
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes AMBRA1 phosphorylated at Ser52.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Bovine, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AMBRA1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa. This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC80 04053252915987

      Documentation

      Anti-phospho AMBRA1 Antibody (Ser52) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho AMBRA1 Antibody (Ser52) Certificates of Analysis

      TitleLot Number
      Anti-phospho AMBRA1 (Ser52) - 2564453 2564453
      Anti-phospho AMBRA1 (Ser52) - 3440802 3440802
      Anti-phospho AMBRA1 (Ser52) - 3590107 3590107
      Anti-phospho AMBRA1 (Ser52) - Q2227355 Q2227355
      Anti-phospho AMBRA1 (Ser52) -2606921 2606921
      Anti-phospho AMBRA1 (Ser52) -2686107 2686107
      Anti-phospho AMBRA1 (Ser52) -2774126 2774126
      Anti-phospho AMBRA1 (Ser52) Polyclonal Antibody 3105787
      Anti-phospho AMBRA1 (Ser52) Polyclonal Antibody 2905400

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies