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101301 Auramine O (C.I. 41000)

for microscopy

Auramine O (C.I. 41000) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Pyoktamin® yellow, 4,4'-Bis-(dimethylamino)-benzophenonimide hydrochloride

101301
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      Description
      Catalogue Number101301
      SynonymsPyoktamin® yellow, 4,4'-Bis-(dimethylamino)-benzophenonimide hydrochloride
      DescriptionAuramine O (C.I. 41000)
      OverviewAuramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
      Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol® also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      CAS number2465-27-2
      EC index number612-097-00-2
      EC number219-567-2
      Hill FormulaC₁₇H₂₂ClN₃
      Chemical formulaC₁₇H₂₂N₃Cl
      Molar Mass303.84 g/mol
      HS Code3212 90 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applications
      Applicationfor microscopy
      Biological Information
      Physicochemical Information
      Melting Point265 °C (decomposition)
      pH value6 - 7 (10 g/l, H₂O, 20 °C)
      Bulk density430 kg/m3
      Solubility10 g/l
      Dimensions
      Materials Information
      Toxicological Information
      LD 50 oralLD50 Rat 1000 mg/kg
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H302: Harmful if swallowed.
      H319: Causes serious eye irritation.
      H351: Suspected of causing cancer.
      H411: Toxic to aquatic life with long lasting effects.
      Precautionary Statement(s)P202: Do not handle until all safety precautions have been read and understood.
      P264: Wash skin thoroughly after handling.
      P273: Avoid release to the environment.
      P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P308 + P313: IF exposed or concerned: Get medical advice/ attention.
      Signal WordWarning
      Storage class10 - 13 Other liquids and solids
      WGKWGK 3 highly hazardous to water
      Disposal3
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Categories of dangerharmful, irritant, carcinogenic, dangerous for the environment
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3077 , 9, III
      Declaration (transport by air) IATA-DGRUN 3077 , 9, III
      Declaration (transport by sea) IMDG-CodeUN 3077 , 9, III, Marine Pollutant: P
      Supplemental Information
      Specifications
      Identity (UV/VIS-Spectrum)passes test
      Absorption maximum λ max (buffer pH 6.0)431 - 433 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 5 %
      Suitability for microscopypasses test
      Global Trade Item Number
      Catalogue Number GTIN
      1013010050 04022536019093