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14-638M MLCK Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.

MLCK Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-638M
250 µg  Also available in 10 μg size (cat#14-638) and in bulk (cat# 14-638-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-638M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMLCK Protein, active, 250 µg
      OverviewN-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged, recombinant, human MLCK, amino acids 1425-1771, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
      Gene Symbol
      • MYLK
      • DKFZp686I10125
      • MLCK108
      • FLJ12216
      • MSTP083
      • KRP
      • MLCK210
      • MLCK
      • Telokin
      Protein TargetMLCK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15746 # Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
      COFACTOR: Magnesium. & Calcium.
      SIZE: 1914 amino acids; 210774 Da
      SUBUNIT: All isoforms including Telokin bind calmodulin.
      TISSUE SPECIFICITY: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
      PTM: MLCK is probably down-regulated by phosphorylation.
      SIMILARITY: SwissProt: Q15746 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 fibronectin type-III domain. & Contains 9 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight43.7kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of ZIPtide (Catalog # 12-545)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-638) and in bulk (cat# 14-638-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-638M 04053252403828

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-638 MLCK Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases