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14-481M PAK2 Protein, active, 250 µg

Active, recombinant human PAK2, residues 3-end, containing N-terminal His6 & T7 & C-terminal His6 tags, for use in Kinase Assays.

PAK2 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-481M
250 µg  Also available in 10 μg size (cat#14-481) and in bulk (cat# 14-481-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-481M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPAK2 Protein, active, 250 µg
      OverviewRecombinant human PAK2, residues 3-end, containing N-terminal His6 and T7, and C-terminal His6 tags
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, recombinant human PAK2, residues 3-end, containing N-terminal His6 & T7 & C-terminal His6 tags, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
      Gene Symbol
      • PAK2
      • PAK65
      • Gamma-PAK
      • hPAK65
      • PAKgamma
      • PAK-2
      Protein TargetPAK2
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13177 # The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase- activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
      SIZE: 524 amino acids; 58043 Da
      SUBUNIT: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with SH3MD4. Interacts with and activated by HIV-1 Nef. PAK-2p34 interacts with ARHGAP10.
      SUBCELLULAR LOCATION: Serine/threonine-protein kinase PAK 2: Cytoplasm. & PAK-2p34: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
      TISSUE SPECIFICITY: Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
      PTM: Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK- 2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate. & During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34. & Ubiquitinated, leading to its proteosomal degradation. & PAK-2p34 is myristoylated.
      SIMILARITY: SwissProt: Q13177 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
      Molecular Weight62.8kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of the Long S6 Kinase substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70 °C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-481) and in bulk (cat# 14-481-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-481M 04053252275692

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-481 PAK2 Protein, active, 10 µg Show Pricing & Availability

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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases