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MAB3510 | Anti-BrdU Antibody, clone BU-1

MAB3510
100 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A FC, ICC, IHC M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMAB3510
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BrdU Antibody, clone BU-1
      Alternate Names
      • BrdU
      Background InformationBromodeoxyuridine (BrdU) is a thymidine analog that, when added exogenously, is incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to label proliferating cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Brdu treated cells
      PresentationHybridoma culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at a final concentration of 10 μg/mL. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/contamination.
      Applications
      ApplicationUse mouse monoclonal Anti-BrdU Antibody, clone BU-1, validated in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry for the detection of bromodeoxyuridine (BrdU).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesAlthough originally reported to stain nuclear DNA BrdU incorporation in ethanol-fixed cells without acid or base denaturation, this property was later attributed to the DNase activity present in a mycoplasma-contaminated BU-1 hybridoma culture supernatant used (Gonchoroff, N.J., et al. (1986). J. Immunol. Methods. 93(1):97-101; Greipp, P.R., et al. (1985). Am. J. Hematol. 20(3):289-292). Please include an appropriate DNA denaturation step during sample preparation prior to immunostaining using this product.
      Biological Information
      ImmunogenBromodeoxyuridine
      EpitopeBrdU
      CloneBU-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityClone BU-1 specifically binds to bromodeoxyuridine with 10% crossreactivity toward iodouridine (10%). Clone BU-1 does not crossreact with fluorodeoxyuridine or any endogenous cellular components such as thymidine or uridine.
      IsotypeIgG2a
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-BrdU Antibody, clone BU-1 -2745643A 2745643A
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2115988 2115988
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2019966 2019966
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2187371 2187371
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2343124 2343124
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - LV1747805 LV1747805
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - NG1867684 NG1867684
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2521466 2521466
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2703711 2703711
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2729779 2729779
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2745643 2745643
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2772671 2772671
      MOUSE ANTI-BROMODEOXYURIDINE (BrdU) MONOCLONAL ANTIBODY - 1951043 1951043

      Referencias bibliográficas | 21 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice.
      Kang, SS; Keasey, MP; Arnold, SA; Reid, R; Geralds, J; Hagg, T
      Neurobiology of disease 49 68-78 2013

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      Serotonin 1A receptor agonist increases species- and region-selective adult CNS proliferation, but not through CNTF.
      Arnold, SA; Hagg, T
      Neuropharmacology 63 1238-47 2012

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      22884499 22884499
      D-Serine regulates proliferation and neuronal differentiation of neural stem cells from postnatal mouse forebrain.
      Xu Huang,Hui Kong,Mi Tang,Ming Lu,Jian-Hua Ding,Gang Hu
      CNS neuroscience & therapeutics 18 2012

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      22280157 22280157
      Foxg1 has an essential role in postnatal development of the dentate gyrus.
      Tian, C; Gong, Y; Yang, Y; Shen, W; Wang, K; Liu, J; Xu, B; Zhao, J; Zhao, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 2931-49 2012

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      What role do annelid neoblasts play? A comparison of the regeneration patterns in a neoblast-bearing and a neoblast-lacking enchytraeid oligochaete.
      Myohara, M
      PloS one 7 e37319 2012

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      Lens regeneration in axolotl: new evidence of developmental plasticity.
      Suetsugu-Maki, R; Maki, N; Nakamura, K; Sumanas, S; Zhu, J; Del Rio-Tsonis, K; Tsonis, PA
      BMC biology 10 103 2012

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      A complement receptor c5a antagonist regulates epithelial to Mesenchymal transition and crystallin expression after lens cataract surgery in Mice.
      Suetsugu-Maki R, Maki N, Fox TP, Nakamura K, Cowper Solari R, Tomlinson CR, Qu H, Lambris JD, Tsonis PA
      Molecular vision 17 949-64. 2011

      Artículo Texto completo
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      The bone morphogenetic protein antagonist noggin protects white matter after perinatal hypoxia-ischemia.
      Dizon ML, Maa T, Kessler JA
      Neurobiol Dis 2011

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      miRNAs in newt lens regeneration: specific control of proliferation and evidence for miRNA networking.
      Nakamura, K; Maki, N; Trinh, A; Trask, HW; Gui, J; Tomlinson, CR; Tsonis, PA
      PloS one 5 e12058 2010

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      Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vivo.
      Maki, N; Suetsugu-Maki, R; Sano, S; Nakamura, K; Nishimura, O; Tarui, H; Del Rio-Tsonis, K; Ohsumi, K; Agata, K; Tsonis, PA
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24 3462-7 2010

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