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MAB374 | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

200 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, R, Rb, F, Fe, Po ELISA, IP, ICC, IF, IHC, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB374
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • G3PDH
      • GAPD
      • OK/SW-cl.12
      • MGC88685
      • G3PD
      • EC
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      Product Information
      • It is expressed in all cells.
      PresentationPurified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
      ApplicationAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesELISA:
      1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

      1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

      Western blot:
      1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

      Immunoaffinity purification of GAPDH.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGlyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Fish
      • Feline
      • Pig
      Species Reactivity NoteHuman, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
      It has been reported that this antibody does not react with GAPDH from E. coli.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Non-Reactive Species
      • E. coli
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
      SIZE: 335 amino acids; 36053 Da
      SUBUNIT: Homotetramer. Binds PRKCI.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
      SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight38 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Glyceraldehyde-3-Phosphate -2792998 2792998
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2145925 2145925
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2388833 2388833
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2430317 2430317
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 1990892 1990892
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2271144 2271144
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2322571 2322571
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2506322 2506322
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1604282 JC1604282
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1641540 JC1641540
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1682928 JC1682928
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1555185 LV1555185
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1581114 LV1581114
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1721601 NG1721601
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1740950 NG1740950
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1741566 NG1741566
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1780785 NG1780785
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1839548 NG1839548
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1857556 NG1857556
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1869834 NG1869834
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2470405 2470405
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2521487 2521487
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2571583 2571583
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2606352 2606352
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2653144 2653144
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2689153 2689153
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2736674 2736674
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5_2842113 2842113

      Referencias bibliográficas | 594 Disponible | Ver todas las referencias

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      Nucleic acids research 43 247-58 2015

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      HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice.
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      Proteotoxic stress induces phosphorylation of p62/SQSTM1 by ULK1 to regulate selective autophagic clearance of protein aggregates.
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      Western Blotting25932647 25932647