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AB5472-50UL | Anti-Glycine Receptor α3 Subunit Antibody

AB5472-50UL
50 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IHC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5472-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glycine Receptor α3 Subunit Antibody
      Alternate Names
      • GLRa3
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      ApplicationThis Anti-Glycine Receptor α3 Subunit Antibody is validated for use in IH for the detection of Glycine Receptor α3 Subunit.
      Key Applications
      • Immunohistochemistry
      Application NotesWestern blot: 1:200 on rat brain membranes.

      Immunohistochemistry on rat brain floating sections and mouse brain floating sections (trypsin pretreatment is recommended).

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA purified peptide corresponding to amino acid residues 355-368 of rat GlyRa3 (Accession P24524).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Glycine receptor alpha3 (GlyRalpha3) protein. The epitope is not similar to any other known proteins.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GLRA3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
      SIZE: 464 amino acids; 53800 Da
      SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity).
      SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Widely distributed throughout the central nervous system.
      DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
      SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
      MISCELLANEOUS: The alpha subunit binds strychnine.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      RABBIT ANTI-GLYCINE RECEPTOR a3 (GlyRa3) -2696619 2696619
      RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) -2532320 2532320
      RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) -2644610 2644610
      RABBIT ANTI-GLYCINE RECEPTOR 3 (GlyR3) -2774103 2774103

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts
      Nikolic, Z, et al
      J Biol Chem, 273:19708-14 (1998) 1998

      9677400 9677400
      Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor
      Kuhse, J, et al
      J Biol Chem, 265:22317-20 (1990) 1990

      2176214 2176214

      Ficha técnica

      Cargo
      RABBIT ANTI-GLYCINE RECEPTOR 3 (GlyR3) AFFINITY PURIFIED POLYCLONAL ANTIBODY