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MAB3448 | Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ELISA, ICC, IHC, IP, WB M Ascites Monoclonal Antibody
      Catalogue NumberMAB3448
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9
      Alternate Names
      • HP1b
      Background InformationChromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

      Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
      Product Information
      • HeLa, 293 or NIH/3T3 cell lysates
      PresentationLiquid. Contains no preservative.
      ApplicationUse Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1:500-1:5,000

      Immunofluorescence: 1:500-1:5,000

      Immunohistochemistry: 1:500-1:5,000

      Immunoprecipitation: 1:500 - 1:5,000

      ELISA: 1:500-1:5,000

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant mouse HP1beta.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with Heterochromatin protein-1 b (HP1b).
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHeterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM]
      Gene Symbol
      • CBX1
      • HP1-BETA
      • HP1B
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
      SIZE: 191 amino acids; 22225 Da
      SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127.
      SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
      PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis.
      SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.
      Molecular Weight28 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - 2395678 2395678
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - 1990890 1990890
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - 2210325 2210325
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - 2327993 2327993
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - 2492103 2492103
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - JBC1778620 JBC1778620
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - JBC1780781 JBC1780781
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - JC1629911 JC1629911
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - LV1512441 LV1512441
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - NG1721845 NG1721845
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - NG1820778 NG1820778
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - NG1853894 NG1853894
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 -2718338 2718338
      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 -2746521 2746521

      Referencias bibliográficas | 36 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Histone H3 lysine 9 trimethylation is required for suppressing the expression of an embryonically activated retrotransposon in Xenopus laevis.
      Herberg, S; Simeone, A; Oikawa, M; Jullien, J; Bradshaw, CR; Teperek, M; Gurdon, J; Miyamoto, K
      Scientific reports 5 14236 2015

      Mostrar resumen
      26387861 26387861
      Dynamic phosphorylation of HP1α regulates mitotic progression in human cells.
      Chakraborty, A; Prasanth, KV; Prasanth, SG
      Nature communications 5 3445 2014

      Mostrar resumen
      Western Blotting24619172 24619172
      HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs.
      Stixová, L; Sehnalová, P; Legartová, S; Suchánková, J; Hrušková, T; Kozubek, S; Sorokin, DV; Matula, P; Raška, I; Kovařík, A; Fulneček, J; Bártová, E
      Epigenetics & chromatin 7 39 2014

      Mostrar resumen
      Western Blotting25587355 25587355
      Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
      Culver-Cochran, AE; Chadwick, BP
      BMC genomics 14 740 2013

      Mostrar resumen
      24168170 24168170
      Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains.
      Takebayashi, S; Lei, I; Ryba, T; Sasaki, T; Dileep, V; Battaglia, D; Gao, X; Fang, P; Fan, Y; Esteban, MA; Tang, J; Crabtree, GR; Wang, Z; Gilbert, DM
      Epigenetics & chromatin 6 42 2013

      Mostrar resumen
      24330833 24330833
      Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis.
      Dowdle, JA; Mehta, M; Kass, EM; Vuong, BQ; Inagaki, A; Egli, D; Jasin, M; Keeney, S
      PLoS genetics 9 e1003945 2013

      Mostrar resumen
      24244200 24244200
      Detection of senescence-associated heterochromatin foci (SAHF).
      Aird, KM; Zhang, R
      Methods in molecular biology (Clifton, N.J.) 965 185-96 2013

      Mostrar resumen
      23296659 23296659
      SOX10 ablation arrests cell cycle, induces senescence, and suppresses melanomagenesis.
      Cronin, JC; Watkins-Chow, DE; Incao, A; Hasskamp, JH; Schönewolf, N; Aoude, LG; Hayward, NK; Bastian, BC; Dummer, R; Loftus, SK; Pavan, WJ
      Cancer research 73 5709-18 2013

      Mostrar resumen
      23913827 23913827
      Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
      Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
      PloS one 8 e58319 2013

      Mostrar resumen
      23505487 23505487
      Developmentally regulated linker histone H1c promotes heterochromatin condensation and mediates structural integrity of rod photoreceptors in mouse retina.
      Popova, EY; Grigoryev, SA; Fan, Y; Skoultchi, AI; Zhang, SS; Barnstable, CJ
      The Journal of biological chemistry 288 17895-907 2013

      Mostrar resumen
      23645681 23645681

      Ficha técnica

      Anti-Heterochromatin Protein-1 beta, clone 1MOD-1A9 - Data Sheet