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MAB1614 | Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

MAB1614
100 µg  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Ma, Sqd, Su, H, M, R ELISA, ICC, IHC, IP, FUNC, RIA, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1614
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
      Background InformationKinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
      Applications
      ApplicationDetect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

      Immunocytochemistry:
      A previous lot of this antibody was used in Immunocytochemistry.

      Immunoprecipitation:
      A previous lot of this antibody was used in Immunoprecipitation.

      EIA/RIA:
      A previous lot of this antibody was used in RIA.

      Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

      Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenBovine brain kinesin.
      Epitopea.a.420-445
      CloneH2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the heavy chain of kinesin near the N-terminus.
      IsotypeIgG2b
      Species Reactivity
      • Chicken
      • Mammals
      • Squid
      • Sea Urchin
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KIF5B
      • UKHC
      • KINH
      • KNS1
      • KNS
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
      SIZE: 963 amino acids; 109685 Da
      SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU.
      SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity).
      DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
      SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain.
      Molecular Weight124 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Kinesin, heavy chain, a.a.420- -2739313 2739313
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 1997923 1997923
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 2069356 2069356
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 2210350 2210350
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1459899 LV1459899
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1594756 LV1594756
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1691087 LV1691087
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1777794 LV1777794
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - NG1842893 NG1842893
      Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - NG1940109 NG1940109
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 - 2446938 2446938
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 - 2491207 2491207
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 -2585813 2585813
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 -2641112 2641112
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 -2676653 2676653
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 -2726771 2726771

      Referencias bibliográficas | 28 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      A method for multiprotein assembly in cells reveals independent action of kinesins in complex.
      Norris, SR; Soppina, V; Dizaji, AS; Schimert, KI; Sept, D; Cai, D; Sivaramakrishnan, S; Verhey, KJ
      The Journal of cell biology 207 393-406 2014

      Mostrar resumen
      25365993 25365993
      Recycling of kinesin-1 motors by diffusion after transport.
      Blasius, TL; Reed, N; Slepchenko, BM; Verhey, KJ
      PloS one 8 e76081 2013

      Mostrar resumen
      24098765 24098765
      Opposing microtubule motors drive robust nuclear dynamics in developing muscle cells.
      Wilson, MH; Holzbaur, EL
      Journal of cell science 125 4158-69 2012

      Mostrar resumen
      22623723 22623723
      RNA interference of Marlin-1/Jakmip1 results in abnormal morphogenesis and migration of cortical pyramidal neurons.
      Ren Vidal,Patricio Fuentes,Jos Valenzuela,Carlos P Alvarado-Diaz,Omar A Ram,Manuel Kukuljan,Andr Couve
      Molecular and cellular neurosciences 51 2012

      Mostrar resumen
      22828129 22828129
      Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H β-synuclein.
      Sekigawa, A; Fujita, M; Sekiyama, K; Takamatsu, Y; Hatano, T; Rockenstein, E; La Spada, AR; Masliah, E; Hashimoto, M
      Molecular brain 5 34 2012

      Mostrar resumen
      23013868 23013868
      A non-mammalian type opsin 5 functions dually in the photoreceptive and non-photoreceptive organs of birds.
      Ohuchi, H; Yamashita, T; Tomonari, S; Fujita-Yanagibayashi, S; Sakai, K; Noji, S; Shichida, Y
      PloS one 7 e31534 2012

      Mostrar resumen
      22348098 22348098
      In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.
      Fort, AG; Murray, JW; Dandachi, N; Davidson, MW; Dermietzel, R; Wolkoff, AW; Spray, DC
      The Journal of biological chemistry 286 22875-85 2011

      Mostrar resumen
      21536677 21536677
      KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development.
      Yu, J; Lei, K; Zhou, M; Craft, CM; Xu, G; Xu, T; Zhuang, Y; Xu, R; Han, M
      Human molecular genetics 20 1061-73 2011

      Mostrar resumen Artículo Texto completo
      21177258 21177258
      Effects of ALS-related SOD1 mutants on dynein- and KIF5-mediated retrograde and anterograde axonal transport.
      Ping Shi,Anna-Lena Ström,Jozsef Gal,Haining Zhu
      Biochimica et biophysica acta 1802 2010

      Mostrar resumen Artículo Texto completo
      20510358 20510358
      A switch in retrograde signaling from survival to stress in rapid-onset neurodegeneration.
      Perlson, E; Jeong, GB; Ross, JL; Dixit, R; Wallace, KE; Kalb, RG; Holzbaur, EL
      The Journal of neuroscience : the official journal of the Society for Neuroscience 29 9903-17 2009

      Mostrar resumen Artículo Texto completo
      19657041 19657041

      Ficha técnica

      Cargo
      Anti-Kinesin, heavy chain, a.a.420-445, clone H2 - Data Sheet