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AB5320 | Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

AB5320
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Mk ELISA, ICC, IHC, IP, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5320
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody
      Alternate Names
      • Chondroitin sulfate proteoglycan NG2
      • Melanoma chondroitin sulfate proteoglycan 3
      • Melanoma-associated chondroitin sulfate proteoglycan
      • chondroitin sulfate proteoglycan 4
      • chondroitin sulfate proteoglycan 4 (melanoma-associated)
      Background InformationNG2 (also known as Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, and Melanoma-associated chondroitin sulfate proteoglycanis) a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
      PresentationPurified rabbit polyclonal in PBS buffer containing 0.02% azide.
      Applications
      ApplicationDetect NG2 Chondroitin Sulfate Proteoglycan using this Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody validated for use in ELISA, IC, IH, IP and WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry:
      A 1:150-1:600 dilution of a previous lot was used in IC.

      Immunohistochemistry:
      1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

      Immunoprecipitation:
      2 μg/mL concentration of a previous lot was used in IP.

      ELISA:
      A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

      Optimal working dilutions and protocols must be determined by end user
      Biological Information
      ImmunogenImmunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Monkey
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      Purification MethodAntibody purified by protein A, then placed over a fibronectin affinity column.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
      SIZE: 2322 amino acids; 250497 Da
      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells
      Molecular Weight270-300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates

      Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-NG2 Chondroitin Sulfate -2664415 2664415
      Anti-NG2 Chondroitin Sulfate -2768460 2768460
      Anti-NG2 Chondroitin Sulfate Proteoglycan 2474971
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2115992 2115992
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2148254 2148254
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2328335 2328335
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2388848 2388848
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2453244 2453244
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2459629 2459629
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 1960853 1960853
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2000807 2000807
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2031329 2031329
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2070826 2070826
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2281665 2281665
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1519145 LV1519145
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1580834 LV1580834
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1634105 LV1634105
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1682694 LV1682694
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1728862 LV1728862
      Anti-NG2 Chondroitin Sulfate Proteoglycan - LV1830075 LV1830075
      Anti-NG2 Chondroitin Sulfate Proteoglycan - NG1853932 NG1853932
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2517782 2517782
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2574604 2574604
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2602767 2602767
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2608781 2608781
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2721718 2721718
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2726769 2726769
      Anti-NG2 Chondroitin Sulfate Proteoglycan -2767726 2767726
      Anti-NG2 Chondroitin Sulfate, Proteoglycan, Polyclonal Antibody 2834672
      SHEEP ANTI-ACTIN, GAMMA -2691400 2691400

      Referencias bibliográficas | 198 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Cytogenesis in the adult monkey motor cortex: perivascular NG2 cells are the major adult born cell type.
      Stanton, GB; Kohler, SJ; Boklweski, J; Cameron, JL; Greenough, WT
      The Journal of comparative neurology 523 849-68 2015

      Mostrar resumen
      25308320 25308320
      Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors.
      Gautier, HO; Evans, KA; Volbracht, K; James, R; Sitnikov, S; Lundgaard, I; James, F; Lao-Peregrin, C; Reynolds, R; Franklin, RJ; Káradóttir, RT
      Nature communications 6 8518 2015

      Mostrar resumen
      26439639 26439639
      3-D Imaging Reveals Participation of Donor Islet Schwann Cells and Pericytes in Islet Transplantation and Graft Neurovascular Regeneration.
      Juang, JH; Kuo, CH; Peng, SJ; Tang, SC
      EBioMedicine 2 109-19 2015

      Mostrar resumen
      26137552 26137552
      Systemic inflammation in early neonatal mice induces transient and lasting neurodegenerative effects.
      Cardoso, FL; Herz, J; Fernandes, A; Rocha, J; Sepodes, B; Brito, MA; McGavern, DB; Brites, D
      Journal of neuroinflammation 12 82 2015

      Mostrar resumen
      25924675 25924675
      The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development.
      Giera, S; Deng, Y; Luo, R; Ackerman, SD; Mogha, A; Monk, KR; Ying, Y; Jeong, SJ; Makinodan, M; Bialas, AR; Chang, BS; Stevens, B; Corfas, G; Piao, X
      Nature communications 6 6121 2015

      Mostrar resumen
      25607655 25607655
      Traumatic brain injury results in rapid pericyte loss followed by reactive pericytosis in the cerebral cortex.
      Zehendner, CM; Sebastiani, A; Hugonnet, A; Bischoff, F; Luhmann, HJ; Thal, SC
      Scientific reports 5 13497 2015

      Mostrar resumen
      26333872 26333872
      Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex.
      Orduz, D; Maldonado, PP; Balia, M; Vélez-Fort, M; de Sars, V; Yanagawa, Y; Emiliani, V; Angulo, MC
      eLife 4 2015

      Mostrar resumen
      25902404 25902404
      A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis.
      Abraham, S; Scarcia, M; Bagshaw, RD; McMahon, K; Grant, G; Harvey, T; Yeo, M; Esteves, FO; Thygesen, HH; Jones, PF; Speirs, V; Hanby, AM; Selby, PJ; Lorger, M; Dear, TN; Pawson, T; Marshall, CJ; Mavria, G
      Nature communications 6 7286 2015

      Mostrar resumen
      26129894 26129894
      Limitations of the dorsal skinfold window chamber model in evaluating anti-angiogenic therapy during early phase of angiogenesis.
      Biel, NM; Lee, JA; Sorg, BS; Siemann, DW
      Vascular cell 6 17 2014

      Mostrar resumen
      25101168 25101168
      Dynamin 2 regulation of integrin endocytosis, but not VEGF signaling, is crucial for developmental angiogenesis.
      Lee, MY; Skoura, A; Park, EJ; Landskroner-Eiger, S; Jozsef, L; Luciano, AK; Murata, T; Pasula, S; Dong, Y; Bouaouina, M; Calderwood, DA; Ferguson, SM; De Camilli, P; Sessa, WC
      Development (Cambridge, England) 141 1465-72 2014

      Mostrar resumen
      24598168 24598168

      Folleto

      Cargo
      SNAP i.d. 2.0 System Brochure

      Licencias necesarias e Información técnica

      Cargo
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Ficha técnica

      Cargo
      RABBIT ANTI-NG2 CHONDROITIN SULFATE PROTEOGLYCAN POLYCLONAL ANTIBODY

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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