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MAB353 | Anti-Nestin Antibody, clone rat-401

100 µg  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R ICC, IHC, IH(P), WB M Purified Monoclonal Antibody
      Catalogue NumberMAB353
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nestin Antibody, clone rat-401
      Alternate Names
      • rat401
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
      Product Information
      • Mouse embryonic brain tissue
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.09% sodium azide.
      ApplicationDetect Nestin using this Anti-Nestin Antibody, clone rat-401 validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blot:
      200-220 kDa in reducing gels of newborn rat or mouse cell extracts. Note that nestin was originally reported (erroneously) to migrate at approximately 440 kDa by SDS-PAGE (Kachinsky, 1995).
      For Westerns, prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -85°C will show breakdown of nestin sometimes into multiple fragments.

      1:20-1:200 dilution of a previous lot was used. Perfuse tissue with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. Suitable for frozen or paraffin-embedded sections.

      1:20-1:200 dilution of a previous lot was used. Fix cells in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenNestin purified from embryonic rat spinal cord.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRodent Nestin protein confirmed with rat and mouse. Rat-401 is not reactive with human nestin protein.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
      Gene Symbol
      • NES
      • ORFNames=Nbla00170
      • FLJ21841
      • Nbla00170
      Non-Reactive Species
      • Human
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
      SIZE: 1621 amino acids; 177439 Da
      SUBUNIT: Interacts with FHOD3 (By similarity).
      TISSUE SPECIFICITY: CNS stem cells.
      DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
      SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      Molecular Weight200-220 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry(paraffin):
      Nestin (cat. # MAB353, Clone 401) staining of Mouse Pancreas, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25 µg/mL, using IHC-Select® detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.
      Optimal Staining of Nestin Monoclonal Antibody: Mouse Pancreas
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information



      IHC Select Paraffin Protocol
      MAB353 IH(P) Summary

      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Nestin, clone rat-401 - 2136619 2136619
      Anti-Nestin, clone rat-401 - 2370131 2370131
      Anti-Nestin, clone rat-401 - 2444141 2444141
      Anti-Nestin, clone rat-401 - LV1739283 LV1739283
      Anti-Nestin, clone rat-401 - 1970329 1970329
      Anti-Nestin, clone rat-401 - 2019929 2019929
      Anti-Nestin, clone rat-401 - 2041569 2041569
      Anti-Nestin, clone rat-401 - 2090128 2090128
      Anti-Nestin, clone rat-401 - 2211943 2211943
      Anti-Nestin, clone rat-401 - 2296728 2296728
      Anti-Nestin, clone rat-401 - 2326984 2326984
      Anti-Nestin, clone rat-401 - 2489064 2489064
      Anti-Nestin, clone rat-401 - LV1575964 LV1575964
      Anti-Nestin, clone rat-401 - LV1634942 LV1634942
      Anti-Nestin, clone rat-401 - LV1714334 LV1714334
      Anti-Nestin, clone rat-401 - LV1732492 LV1732492
      Anti-Nestin, clone rat-401 - LV1770320 LV1770320
      Anti-Nestin, clone rat-401 - LV1770361 LV1770361
      Anti-Nestin, clone rat-401 - LV1797294 LV1797294
      Anti-Nestin, clone rat-401 - NG1848434 NG1848434
      Anti-Nestin, clone rat-401 -2500636 2500636
      Anti-Nestin, clone rat-401 -2610372 2610372
      Anti-Nestin, clone rat-401 -2651930 2651930
      Anti-Nestin, clone rat-401 -2664465 2664465
      Anti-Nestin, clone rat-401 -2710698 2710698
      Anti-Nestin, clone rat-401 -2719703 2719703
      Anti-Nestin, clone rat-401 -2746405 2746405
      Anti-Nestin, clone rat-401 -2780475 2780475
      Anti-Nestin, clone rat-401, Monoclonal Antibody 2824021

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      Dysbindin-1C is required for the survival of hilar mossy cells and the maturation of adult newborn neurons in dentate gyrus.
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      The Journal of biological chemistry 289 29060-72 2014

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      Characterization of ectopic colonies that form in widespread areas of the nervous system with neural stem cell transplants into the site of a severe spinal cord injury.
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      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 14013-21 2014

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      Ficha técnica

      Anti-Nestin, clone rat-401 - Data Sheet