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AB1987 | Anti-Neurofilament M (145 kDa) Antibody, CT

AB1987
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, Po, R, Qu, Rb, Rp ICC, IHC, IH(P), WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1987
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament M (145 kDa) Antibody, CT
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
      References
      Product Information
      FormatSerum
      Control
      • Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
      PresentationNeat rabbit antiserum containing no preservative.
      Applications
      ApplicationAnti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1:200 dilution of a previous lot was used in Immunohistochemistry.
      Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum

      Immunohistochemistry(paraffin):

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityStrong and specific staining for NF-M (145 kDa)
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Pig
      • Rat
      • Quail
      • Rabbit
      • Reptile
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 916 amino acids; 102448 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
      Molecular Weight145 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Neurofilament M (145 kDa), C-terminus - 2387736 2387736
      Anti-Neurofilament M (145 kDa), C-terminus - 2458990 2458990
      Anti-Neurofilament M (145 kDa), -2581779 2581779
      Anti-Neurofilament M (145 kDa), -2649648 2649648
      Anti-Neurofilament M (145 kDa), -2676226 2676226
      Anti-Neurofilament M (145 kDa), -2713261 2713261
      Anti-Neurofilament M (145 kDa), -2736678 2736678
      Anti-Neurofilament M (145 kDa), -2746511 2746511
      Anti-Neurofilament M (145 kDa), -2812040 2812040
      Anti-Neurofilament M (145 kDa), C-terminus - 2073570 2073570
      Anti-Neurofilament M (145 kDa), C-terminus - 2219283 2219283
      Anti-Neurofilament M (145 kDa), C-terminus - JC1634170 JC1634170
      Anti-Neurofilament M (145 kDa), C-terminus - JC1658215 JC1658215
      Anti-Neurofilament M (145 kDa), C-terminus - JC1686000 JC1686000
      Anti-Neurofilament M (145 kDa), C-terminus - LV1520810 LV1520810
      Anti-Neurofilament M (145 kDa), C-terminus - NG1731380 NG1731380
      Anti-Neurofilament M (145 kDa), C-terminus - NG1755001 NG1755001
      Anti-Neurofilament M (145 kDa), C-terminus - NG1815288 NG1815288
      Anti-Neurofilament M (145 kDa), C-terminus - NG1899904 NG1899904

      Referencias bibliográficas | 52 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Why are enteric ganglia so small? Role of differential adhesion of enteric neurons and enteric neural crest cells.
      Rollo, BN; Zhang, D; Simkin, JE; Menheniott, TR; Newgreen, DF
      F1000Research 4 113 2015

      Mostrar resumen
      26064478 26064478
      Glial ankyrins facilitate paranodal axoglial junction assembly.
      Chang, KJ; Zollinger, DR; Susuki, K; Sherman, DL; Makara, MA; Brophy, PJ; Cooper, EC; Bennett, V; Mohler, PJ; Rasband, MN
      Nature neuroscience 17 1673-81 2014

      Mostrar resumen
      25362471 25362471
      A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.
      Ho, TS; Zollinger, DR; Chang, KJ; Xu, M; Cooper, EC; Stankewich, MC; Bennett, V; Rasband, MN
      Nature neuroscience 17 1664-72 2014

      Mostrar resumen
      25362473 25362473
      Ezh2 is required for neural crest-derived cartilage and bone formation.
      Schwarz, D; Varum, S; Zemke, M; Schöler, A; Baggiolini, A; Draganova, K; Koseki, H; Schübeler, D; Sommer, L
      Development (Cambridge, England) 141 867-77 2014

      Mostrar resumen
      24496623 24496623
      Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a.
      Liu, C; Lin, C; Gao, C; May-Simera, H; Swaroop, A; Li, T
      Biology open 3 861-70 2014

      Mostrar resumen
      25190059 25190059
      Progressive disorganization of paranodal junctions and compact myelin due to loss of DCC expression by oligodendrocytes.
      Bull, SJ; Bin, JM; Beaumont, E; Boutet, A; Krimpenfort, P; Sadikot, AF; Kennedy, TE
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 9768-78 2014

      Mostrar resumen
      25031414 25031414
      Slowly emerging glycinergic transmission enhances inhibition in the sound localization pathway of the avian auditory system.
      Fischl, MJ; Weimann, SR; Kearse, MG; Burger, RM
      Journal of neurophysiology 111 565-72 2014

      Mostrar resumen
      Immunohistochemistry24198323 24198323
      Responses of hair follicle-associated structures to loss of planar cell polarity signaling.
      Chang, H; Nathans, J
      Proceedings of the National Academy of Sciences of the United States of America 110 E908-17 2013

      Mostrar resumen
      23431170 23431170
      S[+] Apomorphine is a CNS penetrating activator of the Nrf2-ARE pathway with activity in mouse and patient fibroblast models of amyotrophic lateral sclerosis.
      Mead, RJ; Higginbottom, A; Allen, SP; Kirby, J; Bennett, E; Barber, SC; Heath, PR; Coluccia, A; Patel, N; Gardner, I; Brancale, A; Grierson, AJ; Shaw, PJ
      Free radical biology & medicine 61 438-52 2013

      Mostrar resumen
      Immunohistochemistry23608463 23608463
      Axons are injured by antigen-specific CD8(+) T cells through a MHC class I- and granzyme B-dependent mechanism.
      Sauer, BM; Schmalstieg, WF; Howe, CL
      Neurobiology of disease 59 194-205 2013

      Mostrar resumen
      23899663 23899663

      Ficha técnica

      Cargo
      Anti-Neurofilament M (145 kDa), C-terminus - Data Sheet

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