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MAB5404 | Anti-Nitrotyrosine Antibody, clone 2A8.2

100 µg  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A IH(P), WB M Purified Monoclonal Antibody
      Catalogue NumberMAB5404
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitrotyrosine Antibody, clone 2A8.2
      Background InformationThe cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
      Product Information
      • Nitrated BSA
      PresentationPurified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide.
      ApplicationDetect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenNitrated KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with nitrotyrosine.
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Nitrotyrosine Immunoblot Controls.

      Western Blot Analysis:
      1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Nitrotyrosine, clone 2A8.2 - 2453250 2453250
      Anti-Nitrotyrosine, clone 2A8.2 - 0606033429 0606033429
      Anti-Nitrotyrosine, clone 2A8.2 - 1992645 1992645
      Anti-Nitrotyrosine, clone 2A8.2 - 2073585 2073585
      Anti-Nitrotyrosine, clone 2A8.2 - 2325079 2325079
      Anti-Nitrotyrosine, clone 2A8.2 - LV1434073 LV1434073
      Anti-Nitrotyrosine, clone 2A8.2 - LV1594105 LV1594105
      Anti-Nitrotyrosine, clone 2A8.2 - LV1703543 LV1703543
      Anti-Nitrotyrosine, clone 2A8.2 - LV1736265 LV1736265
      Anti-Nitrotyrosine, clone 2A8.2 - LV1751785 LV1751785
      Anti-Nitrotyrosine, clone 2A8.2 - LV1766466 LV1766466
      Anti-Nitrotyrosine, clone 2A8.2 - LV1777991 LV1777991
      Anti-Nitrotyrosine, clone 2A8.2 -2571198 2571198

      Referencias bibliográficas | 22 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      Tocopherols inhibit oxidative and nitrosative stress in estrogen-induced early mammary hyperplasia in ACI rats.
      Das Gupta, S; So, JY; Wall, B; Wahler, J; Smolarek, AK; Sae-Tan, S; Soewono, KY; Yu, H; Lee, MJ; Thomas, PE; Yang, CS; Suh, N
      Molecular carcinogenesis 54 916-25 2015

      Mostrar resumen
      24782330 24782330
      Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs: is protein S-nitrosylation involved?
      Kovács, M; Kiss, A; Gönczi, M; Miskolczi, G; Seprényi, G; Kaszaki, J; Kohr, MJ; Murphy, E; Végh, Á
      PloS one 10 e0122243 2015

      Mostrar resumen
      25909651 25909651
      Electroacupuncture-like stimulation at the Baihui (GV20) and Dazhui (GV14) acupoints protects rats against subacute-phase cerebral ischemia-reperfusion injuries by reducing S100B-mediated neurotoxicity.
      Cheng, CY; Lin, JG; Tang, NY; Kao, ST; Hsieh, CL
      PloS one 9 e91426 2014

      Mostrar resumen
      24626220 24626220
      Endothelial nitric oxide synthase and superoxide mediate hemodynamic initiation of intracranial aneurysms.
      Liaw, N; Fox, JM; Siddiqui, AH; Meng, H; Kolega, J
      PloS one 9 e101721 2014

      Mostrar resumen
      24992254 24992254
      Targeting immune suppression with PDE5 inhibition in end-stage multiple myeloma.
      Noonan, KA; Ghosh, N; Rudraraju, L; Bui, M; Borrello, I
      Cancer immunology research 2 725-31 2014

      Mostrar resumen
      24878583 24878583
      Amifostine alleviates radiation-induced lethal small bowel damage via promotion of 14-3-3σ-mediated nuclear p53 accumulation.
      Huang, EY; Wang, FS; Chen, YM; Chen, YF; Wang, CC; Lin, IH; Huang, YJ; Yang, KD
      Oncotarget 5 9756-69 2014

      Mostrar resumen
      25230151 25230151
      Vitamin E dietary supplementation improves neurological symptoms and decreases c-Abl/p73 activation in Niemann-Pick C mice.
      Marín, T; Contreras, P; Castro, JF; Chamorro, D; Balboa, E; Bosch-Morató, M; Muñoz, FJ; Alvarez, AR; Zanlungo, S
      Nutrients 6 3000-17 2014

      Mostrar resumen
      Immunohistochemistry25079853 25079853
      A prescribed Chinese herbal medicine improves glucose profile and ameliorates oxidative stress in Goto-Kakisaki rats fed with high fat diet.
      Wu, L; Li, X; Zhu, H; Xu, P; Gao, X
      PloS one 8 e60262 2013

      Mostrar resumen
      23565214 23565214
      GH-independent IGF-I action is essential to prevent the development of nonalcoholic steatohepatitis in a GH-deficient rat model.
      Hitoshi Nishizawa,Michiko Takahashi,Hidenori Fukuoka,Genzo Iguchi,Riko Kitazawa,Yutaka Takahashi
      Biochemical and biophysical research communications 423 2012

      Mostrar resumen
      22659415 22659415
      When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.
      Petre, BA; Ulrich, M; Stumbaum, M; Bernevic, B; Moise, A; Döring, G; Przybylski, M
      Journal of the American Society for Mass Spectrometry 23 1831-40 2012

      Mostrar resumen
      22907170 22907170


      Pathways and Biomarkers of Oxidative Stress

      Licencias necesarias e Información técnica

      White Paper- Modern Methods in Oxidative Stress Research

      Ficha técnica

      Anti-Nitrotyrosine, clone 2A8.2 - Data Sheet