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07-1217 | Anti-PP1β Antibody

07-1217
100 µg  
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      Descripción

      Replacement Information
      Replacement Information07-1217 is a recommended replacement for AB4083

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, R WB, IP, IH(P) Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1217
      ReplacesAB4083
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PP1β Antibody
      Alternate Names
      • Serine/threonine-protein phosphatase PP1-beta catalytic subunit
      • Protein phosphatase 1, catalytic subunit, beta
      • Protein phosphatase 1, catalytic subunit, beta isoform
      • Protein phosphatase 1-beta
      • Protein phosphatase 1-delta
      Background InformationType 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Cell Lysate
      PresentationPurified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
      Applications
      ApplicationThis Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.

      Immunohistochemistry(paraffin):
      Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB.
      Biological Information
      ImmunogenThe immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PPP1CB
      • PP-1B
      • PPP1CD
      • MGC3672
      • EC 3.1.3.16
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62140 # Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 327 amino acids; 37187 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen (By similarity). Interacts with PPP1R7.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity).
      SIMILARITY: SwissProt: P62140 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight~ 38kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.
      Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-PP1 - 2196112 2196112
      Anti-PP1 - 2344724 2344724
      Anti-PP1 - NG1590624 NG1590624
      Anti-PP1 - NG1739933 NG1739933
      Anti-PP1 - NG1800821 NG1800821
      Anti-PP1 - NG1857481 NG1857481
      Anti-PP1 - NG1931494 NG1931494
      Anti-PP1#946; - 1972298 1972298
      Anti-PP1#946; - 2052357 2052357
      Anti-PP1β - 2800519 2800519
      Anti-PP1β -2603239 2603239

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts.
      McCoy, F; Darbandi, R; Chen, SI; Eckard, L; Dodd, K; Jones, K; Baucum, AJ; Gibbons, JA; Lin, SH; Colbran, RJ; Nutt, LK
      The Journal of biological chemistry 288 8838-48 2013

      Mostrar resumen
      23400775 23400775