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07-330 | Anti-Partitioning-defective 3 Antibody

07-330
200 µg  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Mk, M, Ca ICC, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-330
      ReplacesMABS174
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Partitioning-defective 3 Antibody
      Alternate Names
      • Atypical PKC isotype-specific-interacting protein
      • CTCL tumor antigen se2-5
      • atypical PKC isotype-specific interacting protein
      • par-3 (partitioning defective 3, C.elegans) homolog
      • par-3 partitioning defective 3 homolog (C. elegans)
      • partitioning-defective protein 3 homolog
      Background InformationPAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.
      References
      Product Information
      FormatPurified
      Control
      • 3T3/A31 lysate.

        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercapto-ethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-Partitioning-defective 3 Antibody is an antibody against Partitioning-defective 3 and has been published in over 20 citations for use in IC and WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      A previous lot of this antibody showed positive immunostaining for PAR-3 in MDCK cells fixed with 3% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 has been shown by an independent laboratory.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Monkey
      • Mouse
      • Canine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors (GEFs; see MIM 606057) and GTPase-activating proteins (GAPs; see MIM 604980), interacts with a large set of effector proteins that typically contain a CDC42/RAC (MIM 602048)-interactive binding (CRIB) domain.[supplied by OMIM]
      Gene Symbol
      • PAR3
      • PARD3
      • ASIP
      • PHIP
      • SE2-5T2
      • PAR-3
      • SE2-5L16
      • PARD-3
      • PARD3A
      • PAR3A
      • PAR3alpha
      • PAR3-alpha
      • Bazooka
      • FLJ21015
      • Baz
      • SE2-5LT1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8TEW0 # Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
      SIZE: 1356 amino acids; 151423 Da
      SUBUNIT: Interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Interacts with F11R/JAM1 (By similarity). Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cell junction. Cell membrane. Note=Partially localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKCZ.
      SIMILARITY: SwissProt: Q8TEW0 ## Belongs to the PAR3 family. & Contains 3 PDZ (DHR) domains.
      MISCELLANEOUS: Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas.
      Molecular Weight~180, 150 & 100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from 3T3/A31, A431, and HeLa cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this antibody detected PAR-3 isoforms in RIPA lysates from 3T3/A31, A431, and HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-PAR-3 (rabbit polyclonal IgG) - 2193160 2193160
      Anti-Partitioning-defective 3 - 2113356 2113356
      Anti-Partitioning-defective 3 - 2322514 2322514
      Anti-Partitioning-defective 3 - 2391109 2391109
      Anti-Partitioning-defective 3 - 1962116 1962116
      Anti-Partitioning-defective 3 - 2016672 2016672
      Anti-Partitioning-defective 3 - 2044180 2044180
      Anti-Partitioning-defective 3 - DAM1411335 DAM1411335
      Anti-Partitioning-defective 3 - DAM1503384 DAM1503384
      Anti-Partitioning-defective 3 - DAM1597364 DAM1597364
      Anti-Partitioning-defective 3 - DAM1644564 DAM1644564
      Anti-Partitioning-defective 3 - DAM1661072 DAM1661072
      Anti-Partitioning-defective 3 - DAM1690660 DAM1690660
      Anti-Partitioning-defective 3 - DAM1724047 DAM1724047
      Anti-Partitioning-defective 3 - DAM1754 DAM1754
      Anti-Partitioning-defective 3 - DAM1772423 DAM1772423
      Anti-Partitioning-defective 3 - JBC1378227 JBC1378227
      Anti-Partitioning-defective 3 -2488936 2488936
      Anti-Partitioning-defective 3 -2557642 2557642
      Anti-Partitioning-defective 3 -2615671 2615671
      Anti-Partitioning-defective 3 -2717691 2717691
      Anti-Partitioning-defective 3 -2746439 2746439
      Anti-Partitioning-defective 3 -2810719 2810719
      Anti-Rartitioning-defective 3 - 0701050115 0701050115
      Anti-Rartitioning-defective 3 - 0702052407 0702052407
      Anti-Rartitioning-defective 3 - 22308 22308
      Anti-Rartitioning-defective 3 - 28898 28898
      Anti-Rartitioning-defective 3 - A610042442 A610042442

      Referencias bibliográficas | 52 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity.
      Luxenburg, C; Heller, E; Pasolli, HA; Chai, S; Nikolova, M; Stokes, N; Fuchs, E
      Nature cell biology 17 592-604 2015

      Mostrar resumen
      25915128 25915128
      RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
      Kistler, WS; Baas, D; Lemeille, S; Paschaki, M; Seguin-Estevez, Q; Barras, E; Ma, W; Duteyrat, JL; Morlé, L; Durand, B; Reith, W
      PLoS genetics 11 e1005368 2015

      Mostrar resumen
      26162102 26162102
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications 6 8303 2015

      Mostrar resumen
      Immunohistochemistry26383514 26383514
      Par3-mInsc and Gαi3 cooperate to promote oriented epidermal cell divisions through LGN.
      Williams, SE; Ratliff, LA; Postiglione, MP; Knoblich, JA; Fuchs, E
      Nature cell biology 16 758-69 2014

      Mostrar resumen
      25016959 25016959
      Type VII collagen regulates expression of OATP1B3, promotes front-to-rear polarity and increases structural organisation in 3D spheroid cultures of RDEB tumour keratinocytes.
      Dayal, JH; Cole, CL; Pourreyron, C; Watt, SA; Lim, YZ; Salas-Alanis, JC; Murrell, DF; McGrath, JA; Stieger, B; Jahoda, C; Leigh, IM; South, AP
      Journal of cell science 127 740-51 2014

      Mostrar resumen
      24357722 24357722
      Developmental stratification of the mammary epithelium occurs through symmetry-breaking vertical divisions of apically positioned luminal cells.
      Huebner, RJ; Lechler, T; Ewald, AJ
      Development (Cambridge, England) 141 1085-94 2014

      Mostrar resumen
      24550116 24550116
      Dual function of Yap in the regulation of lens progenitor cells and cellular polarity.
      Song, JY; Park, R; Kim, JY; Hughes, L; Lu, L; Kim, S; Johnson, RL; Cho, SH
      Developmental biology 386 281-90 2014

      Mostrar resumen
      24384391 24384391
      PKCζ mediates breakdown of outer blood-retinal barriers in diabetic retinopathy.
      Omri, S; Behar-Cohen, F; Rothschild, PR; Gélizé, E; Jonet, L; Jeanny, JC; Omri, B; Crisanti, P
      PloS one 8 e81600 2013

      Mostrar resumen
      24312324 24312324
      Par-complex aPKC and Par3 cross-talk with innate immunity NF-κB pathway in epithelial cells.
      Forteza, R; Wald, FA; Mashukova, A; Kozhekbaeva, Z; Salas, PJ
      Biology open 2 1264-9 2013

      Mostrar resumen
      24244864 24244864
      Loss of aPKCλ in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains.
      Yamanaka, T; Tosaki, A; Kurosawa, M; Akimoto, K; Hirose, T; Ohno, S; Hattori, N; Nukina, N
      PloS one 8 e84036 2013

      Mostrar resumen
      24391875 24391875

      Productos y aplicaciones relacionados

      Included Positive Control

      Número de referencia Descripción  
      12-305 3T3/A31 Cell Lysate Precios y disponibilidad

      View Gene Related Products for this Target

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies