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05-516 | Anti-Ras Antibody, clone RAS10

05-516
100 µg  
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      Descripción

      Replacement Information
      Replacement Information05-516 is a recommended replacement for CBL751

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M ELISA, FC, ICC, IHC, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-516
      ReplacesCBL751
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ras Antibody, clone RAS10
      Alternate Names
      • GTP- and GDP-binding peptide B
      • GTPase Hras
      • Ha-Ras1 proto-oncoprotein
      • Ras family small GTP binding protein H-Ras
      • Transforming protein p2
      • c-has/bas p21 protein
      • c-ras-Ki-2 activated oncogene
      • p19 H-RasIDX protein
      • transformation gene: oncogene HAMSV
      • v-Ha-ras Harvey rat sarcoma viral oncogene homolog
      Background InformationThe tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels
      PresentationPurified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationDetect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      1-4 μg of this antibody has been reported to immunoprecipitate Ras.

      Immunocytochemistry:
      2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

      Immunohistochemistry:
      This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.
      Biological Information
      ImmunogenPurified recombinant Ras. Clone RAS10.
      CloneRAS10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes p21H-, K- and N-Ras.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteProven to react with human, mouse and rat. Other species unknown.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
      Gene Symbol
      • K-Ras
      • Ki-Ras
      • K-Ras2
      • Kras-2
      • p21B
      • KRAS
      • RASK2
      • HRAS
      • HA_RAS
      • N-RAS
      • H-RAS
      • NRAS
      • NRAS1
      • ALPS4
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
      Enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
      SIZE: 189 amino acids; 21,656 Da
      SUBUNIT: Interacts with PHLPP (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side.
      Involvement in disease:
      Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.

      Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.

      Defects in KRAS are the cause of Noonan syndrome 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant.

      Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.

      KRAS mutations are involved in cancer development.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

      Western Blot Analysis:
      0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Ras, clone RAS10 - 2117168 2117168
      Anti-Ras, clone RAS10 - 2138010 2138010
      Anti-Ras, clone RAS10 - 2455627 2455627
      Anti-Ras, clone RAS10 - 0605030869 0605030869
      Anti-Ras, clone RAS10 - 0610042800 0610042800
      Anti-Ras, clone RAS10 - 0703054346 0703054346
      Anti-Ras, clone RAS10 - 18495 18495
      Anti-Ras, clone RAS10 - 18715 18715
      Anti-Ras, clone RAS10 - 18954 18954
      Anti-Ras, clone RAS10 - 1957270 1957270
      Anti-Ras, clone RAS10 - 19637 19637
      Anti-Ras, clone RAS10 - 1984834 1984834
      Anti-Ras, clone RAS10 - 2032281 2032281
      Anti-Ras, clone RAS10 - 21073 21073
      Anti-Ras, clone RAS10 - 21190 21190
      Anti-Ras, clone RAS10 - 21916 21916
      Anti-Ras, clone RAS10 - 2309055 2309055
      Anti-Ras, clone RAS10 - 23496 23496
      Anti-Ras, clone RAS10 - 2377376 2377376
      Anti-Ras, clone RAS10 - 24034 24034
      Anti-Ras, clone RAS10 - 26154 26154
      Anti-Ras, clone RAS10 - 26679 26679
      Anti-Ras, clone RAS10 - 29897 29897
      Anti-Ras, clone RAS10 - 31846 31846
      Anti-Ras, clone RAS10 - 33438 33438
      Anti-Ras, clone RAS10 - DAM1394761 DAM1394761
      Anti-Ras, clone RAS10 - DAM1432892 DAM1432892
      Anti-Ras, clone RAS10 - DAM1487543 DAM1487543
      Anti-Ras, clone RAS10 - DAM1601822 DAM1601822
      Anti-Ras, clone RAS10 - DAM1655247 DAM1655247
      Anti-Ras, clone RAS10 - DAM1682123 DAM1682123
      Anti-Ras, clone RAS10 - DAM1695856 DAM1695856
      Anti-Ras, clone RAS10 - DAM1739155 DAM1739155
      Anti-Ras, clone RAS10 - DAM1791300 DAM1791300
      Anti-Ras, clone RAS10 - DAM1828786 DAM1828786
      Anti-Ras, clone RAS10 - JBC1375439 JBC1375439
      Anti-Ras, clone RAS10 - JBC1883069 JBC1883069
      Anti-Ras, clone RAS10 - JBC1893981 JBC1893981
      Anti-Ras, clone RAS10 -2484172 2484172
      Anti-Ras, clone RAS10 -2500505 2500505
      Anti-Ras, clone RAS10 -2554098 2554098
      Anti-Ras, clone RAS10 -2668898 2668898
      Anti-Ras, clone RAS10 -2673037 2673037
      Anti-Ras, clone RAS10 -2686887 2686887
      Anti-Ras, clone RAS10 -2691205 2691205
      Anti-Ras, clone RAS10 -2727191 2727191
      Anti-Ras, clone RAS10 -2763999 2763999
      Anti-Ras, clone RAS10 -2811985 2811985
      Anti-Ras, clone RAS10 -2834653 2834653
      nti-Ras, clone RAS10 - DAM1432892 DAM1432892

      Referencias bibliográficas | 53 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      Site-specific processing of Ras and Rap1 Switch I by a MARTX toxin effector domain.
      Antic, I; Biancucci, M; Zhu, Y; Gius, DR; Satchell, KJ
      Nature communications 6 7396 2015

      Mostrar resumen
      26051945 26051945
      EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells.
      Roda, D; Castillo, J; Telechea-Fernández, M; Gil, A; López-Rodas, G; Franco, L; González-Rodríguez, P; Roselló, S; Pérez-Fidalgo, JA; García-Trevijano, ER; Cervantes, A; Zaragozá, R
      PloS one 10 e0130543 2015

      Mostrar resumen
      26110767 26110767
      MEK-dependent negative feedback underlies BCR-ABL-mediated oncogene addiction.
      Asmussen, J; Lasater, EA; Tajon, C; Oses-Prieto, J; Jun, YW; Taylor, BS; Burlingame, A; Craik, CS; Shah, NP
      Cancer discovery 4 200-15 2014

      Mostrar resumen
      24362263 24362263
      MicroRNA-5p and -3p co-expression and cross-targeting in colon cancer cells.
      Choo, KB; Soon, YL; Nguyen, PN; Hiew, MS; Huang, CJ
      Journal of biomedical science 21 95 2014

      Mostrar resumen
      Western Blotting25287248 25287248
      PLC-γ and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras.
      Diaz-Flores, E; Goldschmidt, H; Depeille, P; Ng, V; Akutagawa, J; Krisman, K; Crone, M; Burgess, MR; Williams, O; Houseman, B; Shokat, K; Sampath, D; Bollag, G; Roose, JP; Braun, BS; Shannon, K
      Science signaling 6 ra105 2013

      Mostrar resumen
      24300897 24300897
      Endomembrane H-Ras controls vascular endothelial growth factor-induced nitric-oxide synthase-mediated endothelial cell migration.
      Haeussler, DJ; Pimentel, DR; Hou, X; Burgoyne, JR; Cohen, RA; Bachschmid, MM
      The Journal of biological chemistry 288 15380-9 2013

      Mostrar resumen
      Western Blotting23548900 23548900
      The RasGAP gene, RASAL2, is a tumor and metastasis suppressor.
      McLaughlin, SK; Olsen, SN; Dake, B; De Raedt, T; Lim, E; Bronson, RT; Beroukhim, R; Polyak, K; Brown, M; Kuperwasser, C; Cichowski, K
      Cancer cell 24 365-78 2013

      Mostrar resumen
      24029233 24029233
      Integrative genomic and functional profiling of the pancreatic cancer genome.
      Shain, AH; Salari, K; Giacomini, CP; Pollack, JR
      BMC genomics 14 624 2013

      Mostrar resumen
      24041470 24041470
      Elucidating distinct roles for NF1 in melanomagenesis.
      Maertens, O; Johnson, B; Hollstein, P; Frederick, DT; Cooper, ZA; Messiaen, L; Bronson, RT; McMahon, M; Granter, S; Flaherty, K; Wargo, JA; Marais, R; Cichowski, K
      Cancer discovery 3 338-49 2013

      Mostrar resumen
      Western Blotting23171796 23171796
      Identification of mutant K-Ras-dependent phenotypes using a panel of isogenic cell lines.
      Vartanian, S; Bentley, C; Brauer, MJ; Li, L; Shirasawa, S; Sasazuki, T; Kim, JS; Haverty, P; Stawiski, E; Modrusan, Z; Waldman, T; Stokoe, D
      The Journal of biological chemistry 288 2403-13 2013

      Mostrar resumen
      23188824 23188824

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies