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MAB3242 | Anti-Smoothelin Antibody, clone R4A

MAB3242
100 µg  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, Ch, H, Ht, Mk, Po, Fe WB, ICC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3242
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Smoothelin Antibody, clone R4A
      Background InformationSmoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
      References
      Product Information
      FormatPurified
      PresentationLiquid in buffer (PBS) with 0.1% sodium azide.
      Applications
      ApplicationDetect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot

      Immunocytochemistry

      Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytoskeletal extract of chicken gizzard.
      CloneR4A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Chicken
      • Human
      • Hamster
      • Monkey
      • Pig
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants.
      Gene Symbol
      • SMTN
      • smoothelin
      • Smoothelin.
      • FLJ38597
      • SMSMO
      • FLJ35365
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P53814 # Structural protein (cytoskeletal).
      SIZE: 917 amino acids; 99516 Da
      SUBCELLULAR LOCATION: Cytoplasm. Note=Exhibits a filamentous organization.
      TISSUE SPECIFICITY: Smooth muscle; contractile or vascular (for the long form).
      SIMILARITY: SwissProt: P53814 ## Contains 1 CH (calponin-homology) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-SMOOTHELIN MONOCLONAL ANTIBODY - 2387773 2387773
      MOUSE ANTI-SMOOTHELIN -2568138 2568138
      MOUSE ANTI-SMOOTHELIN -2605424 2605424
      MOUSE ANTI-SMOOTHELIN -2637113 2637113
      MOUSE ANTI-SMOOTHELIN -2651996 2651996
      MOUSE ANTI-SMOOTHELIN -2698448 2698448
      MOUSE ANTI-SMOOTHELIN -2707903 2707903
      MOUSE ANTI-SMOOTHELIN -2726176 2726176
      MOUSE ANTI-SMOOTHELIN -2779172 2779172
      MOUSE ANTI-SMOOTHELIN MONOCLONAL ANTIBODY - 2069939 2069939

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Congestive heart failure effects on atrial fibroblast phenotype: differences between freshly-isolated and cultured cells.
      Dawson, K; Wu, CT; Qi, XY; Nattel, S
      PloS one 7 e52032 2012

      Mostrar resumen
      23251678 23251678
      Kruppel-like factor 5 is required for formation and differentiation of the bladder urothelium.
      Bell, SM; Zhang, L; Mendell, A; Xu, Y; Haitchi, HM; Lessard, JL; Whitsett, JA
      Developmental biology 358 79-90 2011

      Mostrar resumen
      21803035 21803035
      Cross talk between hedgehog and epithelial-mesenchymal transition pathways in gastric pit cells and in diffuse-type gastric cancers.
      Ohta, H; Aoyagi, K; Fukaya, M; Danjoh, I; Ohta, A; Isohata, N; Saeki, N; Taniguchi, H; Sakamoto, H; Shimoda, T; Tani, T; Yoshida, T; Sasaki, H
      British journal of cancer 100 389-98 2009

      Mostrar resumen Artículo Texto completo
      19107131 19107131
      Dexamethasone preventing contractile and cytoskeletal protein changes in the rabbit basilar artery after subarachnoid hemorrhage.
      Philippe Gomis, Yves Roger Tran-Dinh, Christine Sercombe, Richard Sercombe
      Journal of neurosurgery 102 715-20 2005

      Mostrar resumen
      15871515 15871515
      Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype.
      Hector F Simosa, Grace Wang, XinXin Sui, Timothy Peterson, Vinod Narra, Dario C Altieri, Michael S Conte
      Journal of vascular surgery : official publication, the Society for Vascular Surgery [and] International Society for Cardiovascular Surgery, North American Chapter 41 682-90 2005

      Mostrar resumen
      15874934 15874934

      Ficha técnica

      Cargo
      Anti-Smoothelin, clone R4A - Data Sheet