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MAB318 | Anti-Tyrosine Hydroxylase Antibody, clone LNC1

MAB318
100 µL  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Fg, H, M, Mk, R, Lz, Vo, Zebrafish IHC, IH(P), IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB318
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody, clone LNC1
      Alternate Names
      • TH
      • Tyrosine Monooxygenase
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAscites
      Control
      • Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
      PresentationAscites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.
      Applications
      ApplicationDetect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry(paraffin):
      A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

      Immunoprecipitation:
      A previous lot of this antibody was used in IP (Perez, 2002).

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenTyrosine Hydroxylase purified from PC12 cells
      CloneLNC1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
      IsotypeIgG1κ
      Species Reactivity
      • Chicken
      • Frog
      • Human
      • Mouse
      • Monkey
      • Rat
      • Lizard
      • Vole
      • Zebrafish
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight59-63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western blot:
      1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Tyrosine Hydroxylase, clone LNC1 2464515
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2127781 2127781
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2366531 2366531
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2430129 2430129
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2003001 2003001
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2061430 2061430
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2211927 2211927
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2276308 2276308
      Anti-Tyrosine Hydroxylase, clone LNC1 - 2499557 2499557
      Anti-Tyrosine Hydroxylase, clone LNC1 - JBC1780773 JBC1780773
      Anti-Tyrosine Hydroxylase, clone LNC1 - JC1658254 JC1658254
      Anti-Tyrosine Hydroxylase, clone LNC1 - JC1690775 JC1690775
      Anti-Tyrosine Hydroxylase, clone LNC1 - LV1594176 LV1594176
      Anti-Tyrosine Hydroxylase, clone LNC1 - NG1723972 NG1723972
      Anti-Tyrosine Hydroxylase, clone LNC1 - NG1752067 NG1752067
      Anti-Tyrosine Hydroxylase, clone LNC1 - NG1802536 NG1802536
      Anti-Tyrosine Hydroxylase, clone LNC1 - NG1899912 NG1899912
      Anti-Tyrosine Hydroxylase, clone LNC1 -2523803 2523803
      Anti-Tyrosine Hydroxylase, clone LNC1 -2585892 2585892
      Anti-Tyrosine Hydroxylase, clone LNC1 -2618898 2618898
      Anti-Tyrosine Hydroxylase, clone LNC1 -2651993 2651993
      Anti-Tyrosine Hydroxylase, clone LNC1 -2677893 2677893
      Anti-Tyrosine Hydroxylase, clone LNC1 -2716631 2716631
      Anti-Tyrosine Hydroxylase, clone LNC1_2825556 2825556

      Referencias bibliográficas | 227 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      The temporal expression pattern of alpha-synuclein modulates olfactory neurogenesis in transgenic mice.
      Schreglmann, SR; Regensburger, M; Rockenstein, E; Masliah, E; Xiang, W; Winkler, J; Winner, B
      PloS one 10 e0126261 2015

      Mostrar resumen
      25961568 25961568
      Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.
      Bourdenx, M; Dovero, S; Engeln, M; Bido, S; Bastide, MF; Dutheil, N; Vollenweider, I; Baud, L; Piron, C; Grouthier, V; Boraud, T; Porras, G; Li, Q; Baekelandt, V; Scheller, D; Michel, A; Fernagut, PO; Georges, F; Courtine, G; Bezard, E; Dehay, B
      Acta neuropathologica communications 3 46 2015

      Mostrar resumen
      26205255 26205255
      Prefrontal dopamine regulates fear reinstatement through the downregulation of extinction circuits.
      Hitora-Imamura, N; Miura, Y; Teshirogi, C; Ikegaya, Y; Matsuki, N; Nomura, H
      eLife 4 2015

      Mostrar resumen
      26226637 26226637
      Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.
      Jiang, YH; Jiang, P; Yang, JL; Ma, DF; Lin, HQ; Su, WG; Wang, Z; Li, X
      PloS one 10 e0133971 2015

      Mostrar resumen
      26230083 26230083
      Comprehensive functional characterization of murine infantile Batten disease including Parkinson-like behavior and dopaminergic markers.
      Dearborn, JT; Harmon, SK; Fowler, SC; O'Malley, KL; Taylor, GT; Sands, MS; Wozniak, DF
      Scientific reports 5 12752 2015

      Mostrar resumen
      26238334 26238334
      Viable neuronopathic Gaucher disease model in Medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein.
      Uemura, N; Koike, M; Ansai, S; Kinoshita, M; Ishikawa-Fujiwara, T; Matsui, H; Naruse, K; Sakamoto, N; Uchiyama, Y; Todo, T; Takeda, S; Yamakado, H; Takahashi, R
      PLoS genetics 11 e1005065 2015

      Mostrar resumen
      Immunohistochemistry25835295 25835295
      Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration.
      Greferath, U; Anderson, EE; Jobling, AI; Vessey, KA; Martinez, G; de Iongh, RU; Kalloniatis, M; Fletcher, EL
      Frontiers in cellular neuroscience 9 293 2015

      Mostrar resumen
      26283925 26283925
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research 359 393-407 2015

      Mostrar resumen
      Mouse25418135 25418135
      Catecholaminergic innervation of central and peripheral auditory circuitry varies with reproductive state in female midshipman fish, Porichthys notatus.
      Forlano, PM; Ghahramani, ZN; Monestime, CM; Kurochkin, P; Chernenko, A; Milkis, D
      PloS one 10 e0121914 2015

      Mostrar resumen
      25849450 25849450
      A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.
      Chand, AN; Galliano, E; Chesters, RA; Grubb, MS
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 1573-90 2015

      Mostrar resumen
      25632134 25632134

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies