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07-353 | Anti-acetyl-Histone H3 (Lys14) Antibody

07-353
100 µL  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Yeast (S. cerevisiae) WB, ChIP-seq, DB, Mplex, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-353
      Replaces04-1044
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys14) Antibody
      Alternate Names
      • H3K14Ac
      • Histone H3 (acetyl K14)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
      References
      Product Information
      FormatSerum
      Control
      • C6 cell lysate, NIH 3T3 cell lysate, human breast carcinoma and human lung carcinoma.
      PresentationAntiserum containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys14) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14) & has been validated in ChIP, WB, ChIP-seq, DB, Mplex.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Dot Blot
      • Multiplexing
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation (ChIP):
      An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14.

      Beadlyte® Histone-Peptide Specificity Assay:
      1:1000-1:5000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected.
      Biological Information
      ImmunogenOvalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to amino acids 9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.
      Epitopea.a. 9-18
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes acetyl-histone H3 (Lys14), Mr ~17 kDa. An additional unknown protein was detected at Mr ~33 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Yeast (S. cerevisiae)
      Species Reactivity NoteYeast and human. Broad species-cross reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIn acid extracts from sodium butyrate treated HeLa cells.

      Western Blot Analysis:
      A 1:1000-1:5000 dilution of this lot detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-acetyl-Histone H3 (Lys14) (rabbit antiserum) - DAM1462567 DAM1462567
      Anti-acetyl-Histone H3 (Lys14) - 2387522 2387522
      Anti-acetyl-Histone H3 (Lys14) - 2016673 2016673
      Anti-acetyl-Histone H3 (Lys14) - 2073782 2073782
      Anti-acetyl-Histone H3 (Lys14) - 2200932 2200932
      Anti-acetyl-Histone H3 (Lys14) - 22400 22400
      Anti-acetyl-Histone H3 (Lys14) - 2302183 2302183
      Anti-acetyl-Histone H3 (Lys14) - 24662 24662
      Anti-acetyl-Histone H3 (Lys14) - 30020 30020
      Anti-acetyl-Histone H3 (Lys14) - DAM1548623 DAM1548623
      Anti-acetyl-Histone H3 (Lys14) - DAM1734774 DAM1734774
      Anti-acetyl-Histone H3 (Lys14) - DAM1778033 DAM1778033
      Anti-acetyl-Histone H3 (Lys14) - DAM1821133 DAM1821133
      Anti-acetyl-Histone H3 (Lys14) - JBC1884541 JBC1884541
      Anti-acetyl-Histone H3 (Lys14) -2502998 2502998
      Anti-acetyl-Histone H3 (Lys14) -2554903 2554903
      Anti-acetyl-Histone H3 (Lys14) -2593888 2593888
      Anti-acetyl-Histone H3 (Lys14) -2607277 2607277
      Anti-acetyl-Histone H3 (Lys14) -2686926 2686926
      Anti-acetyl-Histone H3 (Lys14) -2727200 2727200
      Anti-acetyl-Histone H3 (Lys14) -2762291 2762291
      Anti-acetyl-Histone H3 (Lys14) -2816727 2816727

      Referencias bibliográficas | 116 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane.
      Jeong, K; Kwon, H; Lee, J; Jang, D; Pak, Y
      Nucleic acids research 43 3114-27 2015

      Mostrar resumen
      25753664 25753664
      Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.
      Su, LC; Deng, B; Liu, S; Li, LM; Hu, B; Zhong, YT; Li, L
      Frontiers in plant science 6 512 2015

      Mostrar resumen
      26217363 26217363
      The complex pattern of epigenomic variation between natural yeast strains at single-nucleosome resolution.
      Filleton, F; Chuffart, F; Nagarajan, M; Bottin-Duplus, H; Yvert, G
      Epigenetics & chromatin 8 26 2015

      Mostrar resumen
      26229551 26229551
      3D-DIP-Chip: a microarray-based method to measure genomic DNA damage.
      Powell, JR; Bennett, MR; Evans, KE; Yu, S; Webster, RM; Waters, R; Skinner, N; Reed, SH
      Scientific reports 5 7975 2015

      Mostrar resumen
      25609656 25609656
      Epigenetics of Notch1 regulation in pulmonary microvascular rarefaction following extrauterine growth restriction.
      Tang, LL; Zhang, LY; Lao, LJ; Hu, QY; Gu, WZ; Fu, LC; Du, LZ
      Respiratory research 16 66 2015

      Mostrar resumen
      26040933 26040933
      A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.
      Karmodiya, K; Pradhan, SJ; Joshi, B; Jangid, R; Reddy, PC; Galande, S
      Epigenetics & chromatin 8 32 2015

      Mostrar resumen
      26388940 26388940
      Panspecies small-molecule disruptors of heterochromatin-mediated transcriptional gene silencing.
      Castonguay, E; White, SA; Kagansky, A; St-Cyr, DJ; Castillo, AG; Brugger, C; White, R; Bonilla, C; Spitzer, M; Earnshaw, WC; Schalch, T; Ekwall, K; Tyers, M; Allshire, RC
      Molecular and cellular biology 35 662-74 2015

      Mostrar resumen
      25487573 25487573
      Nucleosome competition reveals processive acetylation by the SAGA HAT module.
      Ringel, AE; Cieniewicz, AM; Taverna, SD; Wolberger, C
      Proceedings of the National Academy of Sciences of the United States of America 112 E5461-70 2015

      Mostrar resumen
      26401015 26401015
      Acute ethanol alters multiple histone modifications at model gene promoters in the cerebral cortex.
      Finegersh, A; Homanics, GE
      Alcoholism, clinical and experimental research 38 1865-73 2014

      Mostrar resumen
      24942484 24942484
      A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via histone H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions.
      Gilbert, TM; McDaniel, SL; Byrum, SD; Cades, JA; Dancy, BC; Wade, H; Tackett, AJ; Strahl, BD; Taverna, SD
      Molecular & cellular proteomics : MCP 13 2883-95 2014

      Mostrar resumen
      25104842 25104842

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      Cargo
      Histone Modifications