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07-329 | Anti-acetyl-Histone H4 (Lys16) Antibody

07-329
100 µL  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, Mplex, PIA, DB, ChIP-seq, ChIP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-329
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 (Lys16) Antibody
      Alternate Names
      • H4K16Ac
      • Histone H4 (acetyl K16)
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatAffinity Purified
      Control
      • TSA treated NIH/3T3 cells.
      • HeLa acid extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq.
      Key Applications
      • Western Blotting
      • Multiplexing
      • Peptide Inhibition Assay
      • Dot Blot
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431 and NIH/3T3 cells.
      Chromatin Immunoprecipitation (ChIP) Analysis: 1 µg from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in 1 µL of HeLa cell lysate.
      Dot Blot Analysis: A 1:5,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Multiplexing (MPLX): This antibody specifically recognizes histone H4 acetylated on Lys16 by Luminex® assay.
      Peptide Inhibition Assay (PIA): This antibody peptide blocked on HeLa cell extracts.
      ChIP-Seq Analysis: A representative lot of this antibody was used by two independent
      laboratories for ChIP-Seq (Strome and Ahringer Laboratory, UC Santa Cruz/University of
      Cambridge and Mitzi Kuroda Laboratory, Harvard Medical School) See Egelhofer, T.A., et al. (2011).
      Biological Information
      ImmunogenOvalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
      EpitopeLys16
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity Note Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in 10 µg of sodium butyrate treated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8° C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Acetyl-Histone H4 (Lys16) -2574602 2574602
      Anti-Acetyl-Histone H4 (Lys16) -2639926 2639926
      Anti-Acetyl-Histone H4 (Lys16) -2678056 2678056
      Anti-Acetyl-Histone H4 (Lys16) -2716753 2716753
      Anti-Acetyl-Histone H4 (Lys16) -2780498 2780498
      Anti-acetyl-Histone H4 (Lys16) - 2373394 2373394
      Anti-acetyl-Histone H4 (Lys16) - 2452512 2452512
      Anti-acetyl-Histone H4 (Lys16) - 2459616 2459616
      Anti-acetyl-Histone H4 (Lys16) - 2041108 2041108
      Anti-acetyl-Histone H4 (Lys16) - 2073125 2073125
      Anti-acetyl-Histone H4 (Lys16) - 2178420 2178420
      Anti-acetyl-Histone H4 (Lys16) - 22297 22297
      Anti-acetyl-Histone H4 (Lys16) - 2273956 2273956
      Anti-acetyl-Histone H4 (Lys16) - 2277857 2277857
      Anti-acetyl-Histone H4 (Lys16) - 26818 26818
      Anti-acetyl-Histone H4 (Lys16) - 32214 32214
      Anti-acetyl-Histone H4 (Lys16) - DAM1586179 DAM1586179
      Anti-acetyl-Histone H4 (Lys16) - DAM1612187 DAM1612187
      Anti-acetyl-Histone H4 (Lys16) - DAM1675759 DAM1675759
      Anti-acetyl-Histone H4 (Lys16) - DAM1687546 DAM1687546
      Anti-acetyl-Histone H4 (Lys16) - DAM1713219 DAM1713219
      Anti-acetyl-Histone H4 (Lys16) - DAM1745318 DAM1745318
      Anti-acetyl-Histone H4 (Lys16) - DAM1780380 DAM1780380
      Anti-acetyl-Histone H4 (Lys16) - DAM1805250 DAM1805250
      Anti-acetyl-Histone H4 (Lys16) - JBC1355376 JBC1355376
      Anti-acetyl-Histone H4 (Lys16) - JBC1847859 JBC1847859
      Anti-acetyl-Histone H4 (Lys16) - JBC1888188 JBC1888188
      Anti-acetyl-Histone H4 (Lys16) -2506422 2506422

      Referencias bibliográficas | 85 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      TP53 engagement with the genome occurs in distinct local chromatin environments via pioneer factor activity.
      Sammons, MA; Zhu, J; Drake, AM; Berger, SL
      Genome research 25 179-88 2015

      Mostrar resumen
      25391375 25391375
      Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.
      Lim, KK; Ong, TY; Tan, YR; Yang, EG; Ren, B; Seah, KS; Yang, Z; Tan, TS; Dymock, BW; Chen, ES
      Scientific reports 5 14064 2015

      Mostrar resumen
      26369364 26369364
      Ancestral Chromatin Configuration Constrains Chromatin Evolution on Differentiating Sex Chromosomes in Drosophila.
      Zhou, Q; Bachtrog, D
      PLoS genetics 11 e1005331 2015

      Mostrar resumen
      26114585 26114585
      Rational design and validation of a Tip60 histone acetyltransferase inhibitor.
      Gao, C; Bourke, E; Scobie, M; Famme, MA; Koolmeister, T; Helleday, T; Eriksson, LA; Lowndes, NF; Brown, JA
      Scientific reports 4 5372 2014

      Mostrar resumen
      Western BlottingChicken24947938 24947938
      DNA replication and transcription programs respond to the same chromatin cues.
      Lubelsky, Y; Prinz, JA; DeNapoli, L; Li, Y; Belsky, JA; MacAlpine, DM
      Genome research 24 1102-14 2014

      Mostrar resumen
      24985913 24985913
      The chromatin landscape of Drosophila: comparisons between species, sexes, and chromosomes.
      Brown, EJ; Bachtrog, D
      Genome research 24 1125-37 2014

      Mostrar resumen
      24840603 24840603
      MOF-associated complexes ensure stem cell identity and Xist repression.
      Chelmicki, T; Dündar, F; Turley, MJ; Khanam, T; Aktas, T; Ramírez, F; Gendrel, AV; Wright, PR; Videm, P; Backofen, R; Heard, E; Manke, T; Akhtar, A
      eLife 3 e02024 2014

      Mostrar resumen
      24842875 24842875
      Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory.
      Hait, NC; Wise, LE; Allegood, JC; O'Brien, M; Avni, D; Reeves, TM; Knapp, PE; Lu, J; Luo, C; Miles, MF; Milstien, S; Lichtman, AH; Spiegel, S
      Nature neuroscience 17 971-80 2014

      Mostrar resumen
      Western BlottingMouse24859201 24859201
      Neural stem cell differentiation is dictated by distinct actions of nuclear receptor corepressors and histone deacetylases.
      Castelo-Branco, G; Lilja, T; Wallenborg, K; Falcão, AM; Marques, SC; Gracias, A; Solum, D; Paap, R; Walfridsson, J; Teixeira, AI; Rosenfeld, MG; Jepsen, K; Hermanson, O
      Stem cell reports 3 502-15 2014

      Mostrar resumen
      25241747 25241747
      Mof-associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation.
      Ravens, S; Fournier, M; Ye, T; Stierle, M; Dembele, D; Chavant, V; Tora, L
      eLife 3 2014

      Mostrar resumen
      Western Blotting24898753 24898753

      Póster

      Cargo
      Histone Modifications

      Preguntas frecuentes

      PreguntaRespuesta
      What is the concentration of this antibody? We are not able to quantitate antiserum.