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07-626 | Anti-phospho-AMPKα (Thr172) Antibody

07-626
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Eq, Ch, B WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-626
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-AMPKα (Thr172) Antibody
      Alternate Names
      • 5'-AMP-activated protein kinase, catalytic alpha-1 chain
      • AMP -activate kinase alpha 1 subunit
      • AMP-activated protein kinase, catalytic, alpha-1
      • AMPK alpha 1
      • AMPK alpha-1 chain, protein kinase
      • AMP-activated, alpha 1 catalytic subunit
      Background InformationAMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-AMPKα (Thr172) Antibody is an antibody against phospho-AMPKα (Thr172) for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis:
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes AMPKα.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Horse
      • Chicken
      • Bovine
      Species Reactivity NoteProven to react with human. Predicted to react with mouse, rat, chicken, horse, and cow based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PRKAA1
      • MGC57364
      • AMPK
      • AMPK1
      • MGC33776
      • AMPKa1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
      COFACTOR: Magnesium.
      SIZE: 550 amino acids; 62808 Da
      SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
      SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

      Western Blot Analysis: A 1:500 dilution of this antibody detected AMPKα in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Phospho-AMPK#945; (Thr172) - 2121963 2121963
      Anti-Phospho-AMPK#945; (Thr172) - 2135176 2135176
      Anti-Phospho-AMPK#945; (Thr172) - 2139692 2139692
      Anti-Phospho-AMPK#945; (Thr172) - 2144406 2144406
      Anti-Phospho-AMPK#945; (Thr172) - 2151046 2151046
      Anti-Phospho-AMPK#945; (Thr172) - 2166067 2166067
      Anti-Phospho-AMPKa (Thr172) - 2193167 2193167
      Anti-Phospho-AMPKa (Thr172) - 2239806 2239806
      Anti-Phospho-AMPKa (Thr172) - 2275582 2275582
      Anti-Phospho-AMPKa (Thr172) - 2343568 2343568
      Anti-Phospho-AMPKα (Thr172) - 2391413 2391413
      Anti-Phospho-AMPKα (Thr172) - 2445973 2445973
      Anti-Phospho-AMPKα (Thr172) - 2535293 2535293
      Anti-Phospho-AMPKα (Thr172) -2506423 2506423
      Anti-Phospho-AMPKα (Thr172) -2626701 2626701
      Anti-Phospho-AMPKα (Thr172) -2673047 2673047
      Anti-Phospho-AMPKα (Thr172) -2700181 2700181
      Anti-Phospho-AMPKα (Thr172) -2719882 2719882
      Anti-Phospho-AMPKα (Thr172) -2768427 2768427
      Anti-Phospho-AMPK (Thr172) -2794508 2794508
      Anti-phospho-AMPK alpha; (Thr172) - 27073 27073
      Anti-phospho-AMPK alpha; (Thr172) - 30851 30851
      Anti-phospho-AMPKalpha (Thr172) - 31993 31993

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Comparing the effects of nano-sized sugarcane fiber with cellulose and psyllium on hepatic cellular signaling in mice.
      Wang, Zhong Q, et al.
      Int J Nanomedicine, 7: 2999-3012 (2012) 2012

      Mostrar resumen
      22787396 22787396
      Cold tolerance, cold-induced hyperphagia, and nonshivering thermogenesis are normal in α₁-AMPK-/- mice.
      Bauwens, JD; Schmuck, EG; Lindholm, CR; Ertel, RL; Mulligan, JD; Hovis, I; Viollet, B; Saupe, KW
      American journal of physiology. Regulatory, integrative and comparative physiology 301 R473-83 2011

      Mostrar resumen
      Western Blotting21593427 21593427
      Central role of nitric oxide synthase in AICAR and caffeine-induced mitochondrial biogenesis in L6 myocytes.
      McConell, GK; Ng, GP; Phillips, M; Ruan, Z; Macaulay, SL; Wadley, GD
      Journal of applied physiology (Bethesda, Md. : 1985) 108 589-95 2010

      Mostrar resumen
      Western Blotting20044477 20044477
      Dealing with energy demand: the AMP-activated protein kinase.
      Kemp, B E, et al.
      Trends Biochem. Sci., 24: 22-5 (1999) 1999

      Mostrar resumen
      10087918 10087918
      Isoform-specific purification and substrate specificity of the 5'-AMP-activated protein kinase.
      Michell, B J, et al.
      J. Biol. Chem., 271: 28445-50 (1996) 1996

      Mostrar resumen
      8910470 8910470