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07-164 | Anti-phospho-H2A.X (Ser139) Antibody

07-164
200 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, H IP, WB, ChIP-seq Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-164
      ReplacesMABE205
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-H2A.X (Ser139) Antibody
      Alternate Names
      • H2A histone family, member Q
      • histone 2
      • H2ac
      • histone IIa
      • histone cluster 2
      • H2ac
      Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatPurified
      Control
      • Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      Applications
      ApplicationAnti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Application NotesImmunoprecipitation:
      A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
      Epitopea.a. 134-142
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityHistone H2A.X phosphorylated at Ser139.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      • Human
      Species Reactivity NoteHuman; broad species cross-reactivity expected based on conservation of sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • HIST2H2AC
      • H2A-GL101
      • H2AFQ
      • H2A/r
      • H2A/q
      • MGC74460
      • H2A
      Modifications
      • Phosphorylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

      Western Blot Analysis:
      1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-phospho-H2A.X (Ser139) 2474975
      Anti-phospho-H2A.X (Ser139) - 2117166 2117166
      Anti-phospho-H2A.X (Ser139) - 2391108 2391108
      Anti-phospho-H2A.X (Ser139) - 1971847 1971847
      Anti-phospho-H2A.X (Ser139) - 2019724 2019724
      Anti-phospho-H2A.X (Ser139) - 20284 20284
      Anti-phospho-H2A.X (Ser139) - 2108223 2108223
      Anti-phospho-H2A.X (Ser139) - 2167785 2167785
      Anti-phospho-H2A.X (Ser139) - 21870 21870
      Anti-phospho-H2A.X (Ser139) - 2278512 2278512
      Anti-phospho-H2A.X (Ser139) - 23646 23646
      Anti-phospho-H2A.X (Ser139) - 26079 26079
      Anti-phospho-H2A.X (Ser139) - 32827 32827
      Anti-phospho-H2A.X (Ser139) - DAM1416522 DAM1416522
      Anti-phospho-H2A.X (Ser139) - DAM1546024 DAM1546024
      Anti-phospho-H2A.X (Ser139) - DAM1692623 DAM1692623
      Anti-phospho-H2A.X (Ser139) - DAM1718063 DAM1718063
      Anti-phospho-H2A.X (Ser139) - DAM1745 DAM1745
      Anti-phospho-H2A.X (Ser139) - DAM1787877 DAM1787877
      Anti-phospho-H2A.X (Ser139) - JBC1862912 JBC1862912
      Anti-phospho-H2A.X (Ser139) - JBC1922552 JBC1922552
      Anti-phospho-H2A.X (Ser139) -2517830 2517830
      Anti-phospho-H2A.X (Ser139) -2697443 2697443
      Anti-phospho-H2A.X (Ser139) -2707893 2707893
      Anti-phospho-H2A.X (Ser139) -2716684 2716684
      Anti-phospho-H2A.X (Ser139) -2753982 2753982
      Anti-phospho-H2A.X (Ser139) -2794872 2794872

      Referencias bibliográficas | 93 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant.
      Varas, J; Sánchez-Morán, E; Copenhaver, GP; Santos, JL; Pradillo, M
      PLoS genetics 11 e1005301 2015

      Mostrar resumen
      26147458 26147458
      Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers.
      Lambing, C; Osman, K; Nuntasoontorn, K; West, A; Higgins, JD; Copenhaver, GP; Yang, J; Armstrong, SJ; Mechtler, K; Roitinger, E; Franklin, FC
      PLoS genetics 11 e1005372 2015

      Mostrar resumen
      26182244 26182244
      BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.
      Tatsumi, Y; Takano, R; Islam, MS; Yokochi, T; Itami, M; Nakamura, Y; Nakagawara, A
      Cell death & disease 6 e1607 2015

      Mostrar resumen
      25611382 25611382
      Double-strand break repair deficiency in NONO knockout murine embryonic fibroblasts and compensation by spontaneous upregulation of the PSPC1 paralog.
      Li, S; Li, Z; Shu, FJ; Xiong, H; Phillips, AC; Dynan, WS
      Nucleic acids research 42 9771-80 2014

      Mostrar resumen
      Immunofluorescence25100870 25100870
      STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis.
      Fukuda, T; Fukuda, N; Agostinho, A; Hernández-Hernández, A; Kouznetsova, A; Höög, C
      The EMBO journal 33 1243-55 2014

      Mostrar resumen
      24797475 24797475
      BRCA1 pathway function in basal-like breast cancer cells.
      Hill, SJ; Clark, AP; Silver, DP; Livingston, DM
      Molecular and cellular biology 34 3828-42 2014

      Mostrar resumen
      25092866 25092866
      Long-term quiescent fibroblast cells transit into senescence.
      Marthandan, S; Priebe, S; Hemmerich, P; Klement, K; Diekmann, S
      PloS one 9 e115597 2014

      Mostrar resumen
      Western Blotting25531649 25531649
      GSK-3α is a central regulator of age-related pathologies in mice.
      Zhou, J; Freeman, TA; Ahmad, F; Shang, X; Mangano, E; Gao, E; Farber, J; Wang, Y; Ma, XL; Woodgett, J; Vagnozzi, RJ; Lal, H; Force, T
      The Journal of clinical investigation 123 1821-32 2013

      Mostrar resumen
      23549082 23549082
      Differential contribution of HP1 proteins to DNA end resection and homology-directed repair.
      Soria, G; Almouzni, G
      Cell cycle (Georgetown, Tex.) 12 422-9 2013

      Mostrar resumen
      Western Blotting, Immunofluorescence23287531 23287531
      Ketogenic diets enhance oxidative stress and radio-chemo-therapy responses in lung cancer xenografts.
      Allen, BG; Bhatia, SK; Buatti, JM; Brandt, KE; Lindholm, KE; Button, AM; Szweda, LI; Smith, BJ; Spitz, DR; Fath, MA
      Clinical cancer research : an official journal of the American Association for Cancer Research 19 3905-13 2013

      Mostrar resumen
      Western Blotting23743570 23743570

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