A part of Merck

MKI2MAG-94K | MILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay

MKI2MAG-94K
Diseñe y calcule el precio de su kit
Click To Print This Page

Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN) M Mplex Luminex xMAP
Description
Catalogue NumberMKI2MAG-94K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Mouse Kidney Injury Magnetic Bead Panel 2 contains all the components necessary to measure the following five biomarkers in any combination using Luminex® xMAP® technology: Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate.
Background InformationMaintenance of physiological balance in the body is attributed in part to normal kidney function. An altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. There are multiple risk factors for kidney injury including age, hypertension, diabetes mellitus, heart failure and liver failure. On the other hand, drug induced damage to the kidney results from drug excretion. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. Although both are direct measurements of renal function, increases in serum concentrations of these biomarkers occur only after substantial renal injury. Specificity and earlier detection of kidney injury is vital. For improved detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

We provide valuable research assays to investigate multiple biomarkers of kidney injury in mouse urine samples using the Luminex xMAP® instrument platform.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 10
  • Intra-Assay: < 10
Panel TypeMAGNETIC Toxicity
Applications
ApplicationThe analytes available for this multiplex kit are: Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, Osteopontin (OPN) (for urine samples) or Clusterin, Cystatin C, Lipocalin-2/NGAL, Osteopontin (OPN) (for serum/plasma samples).
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay.
• This assay requires 25 µL of 1:1,000 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Mouse
Analytes Available
  • Clusterin
  • Cystatin C
  • EGF
  • Lipocalin-2/NGAL
  • Osteopontin (OPN)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 90% - 99%
Physicochemical Information
Sensitivity
  • 0.005 - 0.190 ng/mL (MinDC+2SD).
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • Clusterin: 0.2 – 250 ng/mL
  • Cystatin C: 0.05 – 50 ng/mL
  • EGF: 0.04 – 40 ng/mL
  • Lipocalin-2/NGAL: 0.005 – 5 ng/mL
  • Osteopontin (OPN): 0.01 – 10 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Mouse Kidney Injury Magnetic Bead Panel 2

Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Licencias necesarias e Información técnica

Cargo
96 well Template 2 Up
96 well Template Single
Quality Control Ranges: Milliplex Mouse Kidney Injury Magnetic Bead Panel 2

Ficha técnica

Cargo
Full Service Custom-built Assays

Póster

Cargo
Multiplex Assays of Fifteen Human Complement Factors
Novel Kidney Injury Biomarker Panels To Detect Nephrotoxicity in Mouse Models
Toxicology in drug discovery and development: Biomarkers and model organisms