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MMMP3MAG-79K | MILLIPLEX MAP Mouse MMP Magnetic Bead Panel 3 - Immunology Multiplex Assay

MMMP3MAG-79K
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Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
MMP-2, MMP-3, MMP-8, proMMP-9, MMP-12 M Mplex Luminex xMAP
Description
Catalogue NumberMMMP3MAG-79K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse MMP Magnetic Bead Panel 3 - Immunology Multiplex Assay
OverviewMILLIPLEX MAP Mouse MMP Magnetic Bead Panel 3, MMMP3MAG-79K, is a 5 plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue culture samples: MMP-2, MMP-3, MMP-8, proMMP-9, and MMP-12.
Background InformationThe MMPs (matrix metalloproteinases), a family of zinc proteases responsible for the breakdown of extracellular matrix (ECM), play a key role in normal physiological processes, such as embryonic development and tissue morphogenesis, tissue and bone remodeling, wound healing, and angiogenesis. These processes rely on MMPs’ role in the cleavage of cell surface receptors, the release of apoptotic ligands, cell proliferation and differentiation, and chemokine activity modulation. Similar in structure, MMPs are synthesized and secreted as inactive pro-enzymes that require proteolytic cleavage for activation. This process can be mediated by serine proteases or other MMPs. An increase in MMP expression occurs in response to a wide range of stimuli, including adhesion molecules, growth factors, cytokines and hormones. Regulation of MMP activity is controlled primarily by TIMPs (tissue inhibitors of metalloproteinases). Therefore, disruption of the MMP/TIMP balance can result in autoimmune disease, arthritis, cardiovascular disease, and tumor growth and metastasis.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay:< 10
  • Inter-assay: < 20
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationMMP Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple MMP biomarkers in mouse serum or plasma samples.
Key Applications
  • Multiplexing
Application Notes• Cell/tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
• Cell/tissue extracts should be done in neutral buffers containing reagents and conditions that do not interfere with assay performance.
• This kit may be used for the analysis of all or any combination of the Mouse MMP Panel 3 analytes in cell/tissue culture supernatant or extracts.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • MMP-2
  • MMP-3
  • MMP-8
  • proMMP-9
  • MMP-12
Physicochemical Information
Sensitivity
  • See protocol for sensitive’s of individual analytes
Standard Curve Range
  • MMP-2: 49 – 200,000 pg/mL
  • MMP-3: 2 – 10,000 pg/mL
  • MMP-8: 12 – 50,000 pg/mL
  • proMMP-9: 12 – 50,000 pg/mL
  • MMP-12: 5 – 20,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Mouse MMP Magnetic Bead Panel 3 --96-Well Plate Assay

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A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

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96 well Template 2 Up
96 well Template Single
MMMP3MAG-79K xPonent Procotol Template Version Magpix
MMMP3MAG-79K xPonent Standard Template Version Magpix
Quality Control Ranges: Mouse MMP Magnetic Bead Panel 1 , Panel 2 and Panel 3

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Full Service Custom-built Assays

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Multiplex Assays of Fifteen Human Complement Factors