A part of Merck

S7151 | OxyBlot™ Protein Standard

150 µL  
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      Replacement Information

      Tabla espec. clave

      Key Applications
      Catalogue NumberS7151
      Brand Family Chemicon®
      Trade Name
      • OxyBlot
      • Chemicon
      DescriptionOxyBlot™ Protein Standard
      OverviewOxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit.

      Intended Use

      OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western transfer and immunodetection steps of the OxyBlot™ procedure.
      Product Information
      • Each vial contains 150 μl of OxyBlot Protein Standard, which is sufficient for 60 minigels.
      ApplicationOxyBlot Protein Standard serves as an internal control for the electrophoresis, western transfer & immunodetection steps of the OxyBlot procedure.
      Key Applications
      • Western Blotting
      Application NotesMaterial Provided:

      Each vial contains 150 μl of OxyBlot™ Protein Standard, which is sufficient for 60 minigels.

      Instructions for Use:

      Warm the mixture to room temperature. (Since the mixture contains SDS that may precipitate during storage, it may be necessary to heat the stock solution to 50°C to redissolve the SDS). Combine 2.5 μl of the OxyBlot™ Protein Standard with 20 μl of 1X Gel Loading Buffer prior to loading onto the minigel. Do not heat prior to gel loading. Perform SDS-PAGE according to standard procedures.

      NOTE: Since the proteins in the standard mixture already contain DNP residues, the protein standard should not be subjected to the derivatization reaction in the OxyBlot™ Protein Oxidation Detection Kit protocol.

      In order to maintain consistency, the 2-Mercaptoethanol must be omitted from the 1X Gel Loading Buffer if a reducing agent was not used in the preparation of the experimental samples.

      Reagent Preparation:

      1X Gel Loading Buffer

      To make 8 ml:

      0.5 M Tris-HCl, pH 6.8 1.0 ml

      Glycerol 0.8 ml

      14.3 M 2-Mercaptoethanol (Reagent Grade) 0.1 ml

      10% SDS 1.6 ml

      0.05% Bromophenol Blue 0.32 ml

      dH2O 4.18 ml

      Divide into 1 ml aliquots and store at -15°C to -25°C.

      Protein Molecular Weight

      Phosphorylase B 97,400

      Bovine serum albumin 68,000

      Ovalbumin 43,000

      Carbonic anhydrase 29,000

      Trypsin inhibitor 21,000

      OxyBlot™ is a trademark of Serologicals Company
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPrior to first use, store at 4°C. After using for the first time, divide the remaining mixture among several tubes. Store the aliquots at -15°C to -25°C. Dividing the mixture into aliquots serves to protect the mixture from excessive freeze-thaw cycles.
      Packaging Information
      Material Size150 µL
      Transport Information
      Supplemental Information