Guava® easyCyte Instrument Performance |
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The Guava® easyCyte format is compact and offers many new innovations in optical performance and software.
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8 Peak Rainbow Beads
How it Works
The Guava® easyCyte systems use patented, microcapillary, laser-based technology capable of detecting mammalian and microbial cells and beads. A sample of fluorescently labeled cells is aspirated into a uniquely proportioned microcapillary flow cell. Forward and side scatter characteristics are detected by photodiode, and fluorophores excited by the violet, blue, or red laser emit signals that are spectrally filtered to resolve up to 10 fluorophores simultaneously.Instrument Accuracy
Accuracy on the Guava® easyCyte 8HT is shown using a human whole blood cocktail (FlowCellectTM Human CD4/CD8 T Cell Kit). Results were comparable to a predicate method on a sheath-fluid based flow cytometer.Accuracy on the easyCyte™ 8HT is comparable to traditional flow cytometry
Superior small particle bead detection down to 0.2 μm now on the Guava® easyCyte 12 instruments.
Consistent Sample Results Across an Entire Plate with Sample Mixing
- To ensure that cells stay in suspension during sampling, a rotating, variable- speed mixing paddle with auto-cleaning is used between each well so there is no need for manual resuspension after loading the plate.
Evaluation of well-to-well consistency with sample mixing for a 96-well plate.
Sample concentrations were within acceptable range for all samples within the plate.
easyCheck beads were diluted at 1.0x10e6 and placed into all wells of a 96 well plate.
The plate was then spun at 150xg for 5 minutes to mimic settling.
Each well was acquired and concentrations were determined.
Data show the concentrations for each well (solid line) as well as +/- 10% values (dotted lines)
- The mixing feature virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats. Data shown below confirms that carryover is minimized with this format
| Plate 1 | Plate 2 | Plate 3 | Plate 4 | Plate 5 | |
|---|---|---|---|---|---|
| Carryover | 0.15% ± | 0.17% ± | 0.21% ± | 0.23% ± | 0.22% ± |
| Percentage | 0.01 | 0.17 | 0.08 | 0.07 | 0.04 |
Consistent Cell Counts for Each Channel
Fluorescence resolution for all channels show consistent counts and population percentages, for whole blood
Scatter Resolution for 8-Color Staining
| Laser | Channel | Count | % of Population |
|---|---|---|---|
| 488 nm Laser | FITC | 1099 | 9.9 |
| PE | 1205 | 10.6 | |
| PE-Cy5.5 | 1175 | 10.4 | |
| PE-Cy7 | 1085 | 9.6 | |
| 635 nm Laser | APC | 1239 | 11.1 |
| APC-Cy7 | 1131 | 10.0 |
Immunological Phenotyping 6-Color Assay
The Guava® easyCyte 8HT system accurately discriminates six different fluorochromes within a single assay.As shown in the data below, the percentages and absolutely counts of six different populations were evaluated from whole blood.
5 Decade Dynamic Range Enables Simultaneous Detection of Bright and Dim Fluorochromes
HEK-293 cells transfected with GFP (green) or untransfected (grey).
- High calibration mode enhances the detection of very dim fluorochromes, as it improves the signal to noise ratio, even in the red and far-red (APC) channel.
- Positive and negative populations are detectable in the same scale, even for ultrabright fluorochrome dyes or fluorescent proteins, as shown here.
Software Templates for Simple Acquisition and Analysis
- InCyte™ templates are new applications-specific modules with unique features that can be customized to your preferences for analysis. These modules are available for new and existing FlowCellect™ applications.
Pre-Calibrated Instruments that Need No Adjustment
- Every Guava® easyCyte instrument manufactured now has optimized calibrated performance to ensure instrument to instrument reproducibility and performance.
- No user adjustments are necessary, as laser and optical filter alignments are fixed with buffers in place to reduce the effects of vibration transference from internal mechanisms or external sources.
Transfer Methods and Setting Between Any Guava® easyCyte, Even for Systems with Different Optical Layouts
- The ability to transfer instrument settings and methods between instruments allows comparisons of data between instruments and cross-site validation studies.
- Fully-adjustable instrument settings on Guava® easyCyte flow cytometers and GuavaSoft™ software allow the user to optimize, save, and retrieve acquisition settings for SSc and all fluorescent channels (except FSc and NIR2, available late 2012) and display templates.
Over 16 Years of Experience in Microcapillary Flow Cytometry
Since 1998, guava systems have helped many scientists achieve insightful cellular analysis. A summary of the patent history associated with microcapillary technology is listed here:The Guava® easyCyte HT System is the subject of issued and pending US patents and foreign equivalents, including the following US patents:
- 5,798,222 – Apparatus for monitoring substances in organisms (1998)
- 6,403,378 – Cell Viability assay reagents (2002)
- 6,710,871 and 6,816,257 – Method and apparatus for detecting microparticles in fluid samples (2004)
- 7,320,775 – Exchangeable flow cell assembly with a suspended capillary (2008)
- 7,410,809 – Particle or cell analyzer and method (2008)
- 8,184,271 and 7,847,923 – Differentiation of flow cytometry pulses and applications (2010)