White Functionalized Microspheres
Functionalized White Estapor® Microspheres |
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Highly polar or ionizable chemical groups increase the colloidal stability of the microspheres suspension and allow covalent binding of polyclonal or monoclonal antibodies, proteins and haptens. In comparison to physical adsorption, covalent binding of antigens or antibodies to polymer microspheres improves the test performance, and the reagents produced are more stable over time.
Moreover, microspheres with specific functional groups on their surface can bind proteins covalently in the appropriate orientation, directly or by additional activation, giving more sensitive and specific immunoreagents. Some of the surface groups able to covalently bound directly amino acid protein residues are chloromethyl; or after preactivation with carboxy, amino or hydroxy groups.
Due to their more hydrophilic surface, our functionalized polymer microspheres have very low non-specific binding, and a better signal-to-noise ratio.
Carboxyl–modified white microspheres (–COOH)
Highly polar or ionisable chemical groups increase latex stability and also facilitate covalent coupling of proteins to the microsphere surface.The groups –COOH are produced by copolymerisation of the corresponding functional monomers. For carboxylated latexes, eliminating surfactant and lowering pH decrease latex stability and may trigger partial flocculation. In this case, we recommend diluting the latex further and slowing the stirring. If the result is still unsatisfactory, a non-ionic emulsifier may be added. (0.1 to 0.5 g/L of TWEEN 20).
Amino–modified white microspheres (–NH2)
Highly polar or ionisable chemical groups increase latex stability and also facilitate covalent coupling of proteins to the microsphere surface.The groups –NH2 are produced by copolymerisation of the corresponding functional monomers.
Chloromethyl–modified white microspheres (–CH2Cl)
Highly polar or ionisable chemical groups increase latex stability and also facilitate covalent coupling of proteins to the microsphere surface.The groups –CH2Cl are produced by copolymerisation of the corresponding functional monomers.
Hydroxy-modified White Estapor® Microspheres (–OH)
Highly polar or ionisable chemical groups increase latex stability and also facilitate covalent coupling of proteins to the microsphere surface.The groups –OH are produced by copolymerisation of the corresponding functional monomers.
