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FAK100 Actin Cytoskeleton / Focal Adhesion Staining Kit

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FAK100
100 assays  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey Applications
      B, H, Po, RbICC
      Description
      Catalogue NumberFAK100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionActin Cytoskeleton / Focal Adhesion Staining Kit
      OverviewThe Actin Cytoskeleton and Focal Adhesion Staining Kit consists of three components (TRITC-conjugated phalloidin, anti-Vinculin and DAPI) for the immunofluorescent staining of actin filaments in the cytoskeleton, focal contacts as well as the nucleus of the cells.

      Reagents and materials supplied in the Actin Cytoskeleton and Focal Adhesion Staining Kit are sufficient for 100 tests (including controls).

      Introduction

      The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The functions of the actin cytoskeleton is to mediate a variety of essential biological functions in all eukaryotic cells, including intra- and extra-cellular movement and structural support. To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially. Many proteins associated with the actin cytoskeleton are thus likely targets of signaling pathways controlling actin assembly. Actin cytoskeleton assembly is regulated at multiple levels, including the organization of actin monomers (G-protein) into actin polymers and the superorganization of actin polymers into a filamentous network (F-actin - the major constituent of microfilaments) [1]. This superorganization of actin polymers into a filamentous network is mediated by actin side-binding or cross-linking proteins [2, 3, 4]. Actin cytoskeleton is a dynamic structure that rapidly changes shape and organization in reponse to stimuli and cell cycle progression. Therefore, a disruption of its normal regulation may lead to cell transformations, hence cancer. Transformed cells have been shown to contain less F-actin than untransformed cells and exhibit atypical coordination of F-actin levels to the cell cycle [5]. Orientational distribution of actin filaments within a cell is an important determinant of cellular shape and motility. Therefore,

      Focal adhesion and adherens junctions are membrane-associated complexes that serve as nucleation sites for actin filaments and as cross-linkers between the cell exterior, plasma membrane and actin cytoskeleton [6]. The function of focal adhesions are structural, linking the ECM on the outside to the actin cytoskeleton on the inside. They are also sites of signal transduction, initiating of signaling pathways in response to adhesion. Focal adhesions consist of integrin-type receptors that are attached to the extracellular matrix and are intracellularly associated with protein complexes containing vinculin (universal focal adhesion marker), talin, a-actinin, paxillin, tensin, zyxin and focal adhesion kinase (FAK) [7,8].

      CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit (Catalog number FAK100) is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
      Materials Required but Not Delivered1. Fixative, e.g. 4% paraformaldehyde

      2. Permeabilizing reagent, e.g. 0.1% Triton X-100

      3. Blocking solution (1% BSA in 1xPBS)

      4. Fluorescent-labeled Secondary antibodies (For example CHEMICON Cat. No. AP124F)

      5. 1 x Wash Buffer (e.g. 1xPBS containing 0.05% Tween-20)

      6. Antifade mounting solution (For example: CHEMICON Cat. No. 5013)

      7. Glass slides and coverslips
      References
      Product Information
      Components
      • Vinculin Monoclonal antibody, purified clone 7F9 (Part No. 90227). One vial containing 100 μL at 1 mg/mL.
      • TRITC-conjugated Phalloidin (Part No. 90228). One vial containing 15 μg.
      • DAPI (Part No. 90229). One vial containing 100 μL.
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationThe Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & DAPI for the immunofluorescent staining of actin filaments in the cytoskeleton as well as the nucleus of the cells.
      Key Applications
      • Immunocytochemistry
      Biological Information
      HostMouse
      Species Reactivity
      • Bovine
      • Human
      • Pig
      • Rabbit
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      FAK100 04053252468711

      Documentation

      Actin Cytoskeleton / Focal Adhesion Staining Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Referencias bibliográficas

      Visión general referenciasPub Med ID
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      Young Suk Jung,Xu-Wen Liu,Rosemarie Chirco,Richard B Warner,Rafael Fridman,Hyeong-Reh Choi Kim
      PloS one  7  2011

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      Pharmaceutical research  28  2010

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      Mitochondria-targeted peptide MTP-131 alleviates mitochondrial dysfunction and oxidative damage in human trabecular meshwork cells.
      Min Chen,Bingqian Liu,Qianying Gao,Yehong Zhuo,Jian Ge
      Investigative ophthalmology & visual science  52  2010

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      Manipulating location, polarity, and outgrowth length of neuron-like pheochromocytoma (PC-12) cells on patterned organic electrode arrays.
      Yu-Sheng Hsiao,Chung-Chih Lin,Hsin-Jui Hsieh,Shih-Min Tsai,Chiung-Wen Kuo,Chih-Wei Chu,Peilin Chen
      Lab on a chip  11  2010

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      How to assess cytotoxicity of (iron oxide-based) nanoparticles: a technical note using cationic magnetoliposomes.
      Stefaan J H Soenen,Marcel De Cuyper
      Contrast media & molecular imaging  6  2010

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      Balance of life and death in alveolar epithelial type II cells: proliferation, apoptosis, and the effects of cyclic stretch on wound healing.
      Lynn M Crosby,Charlean Luellen,Zhihong Zhang,Larry L Tague,Scott E Sinclair,Christopher M Waters
      American journal of physiology. Lung cellular and molecular physiology  301  2010

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      Inhibited cell spreading on polystyrene nanopillars fabricated by nanoimprinting and in situ elongation.
      Hu W, Crouch AS, Miller D, Aryal M, Luebke KJ
      Nanotechnology  21  385301. Epub 2010 Aug 26.  2009

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      In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium.
      F Ravanetti, P Borghetti, E De Angelis, R Chiesa, FM Martini, C Gabbi, A Cacchioli
      Acta biomaterialia  6  1014-24  2009

      Mostrar resumen
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      Cell culture and motility study on a polymer surface with a nanometer-scaled stripe structure.
      Sakamoto Y, Sato K, Abo M, Tsukahara T, Kitamori T, Abe K, Yoshimura E
      Biosci Biotechnol Biochem  74  569-72. Epub 2010 Mar 7.  2009

      Mostrar resumen
      20208350 20208350

      Ficha técnica

      Cargo
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      Manuales del usuario

      Cargo
      Actin Cytoskeleton and Focal Adhesion Staining Kit

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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      Categorías

      Life Science Research > Antibodies and Assays > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents
      Life Science Research > Antibodies and Assays > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents