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05-117 Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

500 µg  
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      Replacement Information

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, RNEUT, RIAMPurifiedMonoclonal Antibody
      Catalogue Number05-117
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1
      Alternate Names
      • Basic fibroblast growth factor
      • basic fibroblast growth factor bFGF
      • fibroblast growth factor 2
      • fibroblast growth factor 2 (basic)
      • heparin-binding growth factor 2.
      Background InformationBasic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family.
      In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
      It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.
      Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.
      Product Information
      • Fetal liver or kidney tissue.
      PresentationPurified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.
      Quality LevelMQ100
      ApplicationAnti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 is an antibody against FGF-2/basic FGF (neutralizing) for use in NEUT, RIA.
      Key Applications
      • Neutralizing
      • Radioimmunoassay
      Applications Not Recommended
      • Western Blotting
      Biological Information
      ImmunogenPurified bovine brain basic FGF (HBGF-2). Clone bFM-1.
      Epitopea.a. 5-24
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteThis antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
      Gene Symbol
      • FGF2
      • Prostatropin
      • BFGF
      • prostatropin
      • HBGF-2
      • FGFB
      Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09038 # The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
      SIZE: 155 amino acids; 17254 Da
      SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2.
      SIMILARITY: SwissProt: P09038 ## Belongs to the heparin-binding growth factors family.
      MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. ... hide » see more »
      Molecular Weight17.5 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

      Neutralization of bFGF Activity Assay:
      2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information


      Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 Certificados de análisis

      CargoNúmero de lote
      Anti-FGF-2/basic FGF (neutralizing), -2762408 2762408
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 (mouse monoclonal IgG1k) - DAM1400105 DAM1400105
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2117974 2117974
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2135089 2135089
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2397053 2397053
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1940140 1940140
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1980376 1980376
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 1997385 1997385
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2004527 2004527
      Anti-FGF-2/basic FGF (neutralizing), clone bFM-1 - 2036078 2036078

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF.
      Fessler, E; Borovski, T; Medema, JP
      Molecular cancer  14  157  2015

      Mostrar resumen
      26282129 26282129
      High molecular weight fibroblast growth factor-2 in the human heart is a potential target for prevention of cardiac remodeling.
      Santiago, JJ; McNaughton, LJ; Koleini, N; Ma, X; Bestvater, B; Nickel, BE; Fandrich, RR; Wigle, JT; Freed, DH; Arora, RC; Kardami, E
      PloS one  9  e97281  2014

      Mostrar resumen
      Western Blotting24827991 24827991
      Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma growth.
      Knelson, EH; Gaviglio, AL; Nee, JC; Starr, MD; Nixon, AB; Marcus, SG; Blobe, GC
      The Journal of clinical investigation  124  3016-31  2014

      Mostrar resumen
      24937430 24937430
      Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma.
      Knelson, EH; Gaviglio, AL; Tewari, AK; Armstrong, MB; Mythreye, K; Blobe, GC
      The Journal of clinical investigation  123  4786-98  2013

      Mostrar resumen
      24216509 24216509
      EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1.
      Sáinz-Jaspeado, M; Huertas-Martinez, J; Lagares-Tena, L; Martin Liberal, J; Mateo-Lozano, S; de Alava, E; de Torres, C; Mora, J; Del Muro, XG; Tirado, OM
      PloS one  8  e71449  2013

      Mostrar resumen
      23951165 23951165
      Preferential accumulation and export of high molecular weight FGF-2 by rat cardiac non-myocytes.
      Santiago, JJ; Ma, X; McNaughton, LJ; Nickel, BE; Bestvater, BP; Yu, L; Fandrich, RR; Netticadan, T; Kardami, E
      Cardiovascular research  89  139-47  2010

      Mostrar resumen
      Western Blotting20696821 20696821
      A novel mechanism of sequestering fibroblast growth factor 2 by glypican in lipid rafts, allowing skeletal muscle differentiation.
      Jaime Gutiérrez,Enrique Brandan
      Molecular and cellular biology  30  2009

      Mostrar resumen Artículo Texto completo
      20100867 20100867
      Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells.
      Candela, Maria Elena, et al.
      J. Cell. Physiol., 224: 144-51 (2010)  2009

      Mostrar resumen
      20232295 20232295
      Fibroblast growth factor-2 is a downstream mediator of phosphatidylinositol 3-kinase-Akt signaling in 14,15-epoxyeicosatrienoic acid-induced angiogenesis.
      Zhang, B; Cao, H; Rao, GN
      The Journal of biological chemistry  281  905-14  2005

      Mostrar resumen
      16286479 16286479
      Postischemic intraventricular administration of FGF-2 expressing adenoviral vectors improves neurologic outcome and reduces infarct volume after transient focal cerebral ischemia in rats.
      Watanabe, T; Okuda, Y; Nonoguchi, N; Zhao, MZ; Kajimoto, Y; Furutama, D; Yukawa, H; Shibata, MA; Otsuki, Y; Kuroiwa, T; Miyatake, S
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  24  1205-13  2004

      Mostrar resumen
      15545913 15545913


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      Life Science Research > Antibodies and Assays > Primary Antibodies