Millipore Sigma Vibrant Logo

07-1387 Anti-oxidized-CaM Kinase II (Met281/282) Antibody

This Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: CAMKK, CAMKKB, EC 2.7.11.17 , KIAA0787, MGC15254 UniProt Number: Q96RR4
View Products on Sigmaaldrich.com
07-1387
100 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Ofertas especiales

      100% Performance Guaranteed

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1387
      Replaces04-1079
      DescriptionAnti-oxidized-CaM Kinase II (Met281/282) Antibody
      Alternate Names
      • CAMKK beta protein
      • CaM-KK beta
      • CaM-kinase kinase beta
      • CaMKK beta
      • Calcium/calmodulin-dependent protein kinase kinase beta
      • calcium/calmodulin-dependent protein kinase beta
      • calcium/calmodulin-dependent protein kinase kinase 2 beta
      • calcium/calmodulin-dependent protein kinase kinase 2, beta
      Background InformationThe Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
      References
      Product Information
      FormatSerum
      HS Code3002 15 90
      PresentationUnpurified rabbit serum containing 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesAn independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).
      Biological Information
      ImmunogenSynthetic peptide encompassing and including oxidized Met281/282.
      EpitopeOxidized Met281/282
      HostRabbit
      SpecificityThis antibody recognizes CaM Kinase II when oxidized on Met281/282.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman. Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have
      different expression patterns.
      Gene Symbol
      • CAMKK
      • CAMKKB
      • EC 2.7.11.17
      • KIAA0787
      • MGC15254
      UniProt Number
      UniProt SummaryFUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1 By similarity.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      ENZYME REGULATION: Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca2+/calmodulin. In part, activity is independent on Ca2+/calmodulin By similarity.

      SUBUNIT STRUCTURE: Interacts with calmodulin By similarity.

      SUBCELLULAR LOCATION: CytoplasmBy similarity.

      TISSUE SPECIFICITY: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.

      DOMAIN: The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.

      The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates By similarity.

      PTM: Autophosphorylated By similarity.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

      Contains 1 protein kinase domain.

      SEQUENCE CAUTION: The sequence CAD38990.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 years at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      07-1387 04053252403118

      Documentation

      Anti-oxidized-CaM Kinase II (Met281/282) Antibody Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-oxidized-CaM Kinase II (Met281/282) Antibody Certificados de análisis

      CargoNúmero de lote
      Anti-oxidized-CaM Kinase II (Met281/282) - 3064836 3064836
      Anti-oxidized-CaM Kinase II (Met281/282) - 3197648 3197648
      Anti-oxidized-CaM Kinase II (Met281/282) - 3407316 3407316
      Anti-oxidized-CaM Kinase II (Met281/282) - 3601732 3601732
      Anti-oxidized-CaM Kinase II (Met281/282) - 3762555 3762555
      Anti-oxidized-CaM Kinase II (Met281/282) - 3840864 3840864
      Anti-oxidized-CaM Kinase II (Met281/282) - 3949814 3949814
      Anti-oxidized-CaM Kinase II (Met281/282) - 4074836 4074836
      Anti-oxidized-CaM Kinase II (Met281/282) - 4209964 4209964
      Anti-oxidized-CaM Kinase II (Met281/282) - 4362241 4362241
      Show more