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IPFL00010 Membrana Immobilon-FL, PVDF; 0,45 µm, rollo de 26,5 cm x 3,75 m

Transferencia pulg.Southern pulg., transferencia en pocillos ( pulg.dot/slot pulg.)

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Aplicaciones clave: Western Blotting, Dot Blot
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IPFL00010
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      Descripción

      Replacement Information

      Tabla espec. clave

      Aplicaciones clave
      WB, DB
      Descripción
      Número de catálogoIPFL00010
      Nombre comercial
      • Immobilon
      DescripciónMembrana Immobilon-FL, PVDF; 0,45 µm, rollo de 26,5 cm x 3,75 m
      Información preliminarOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      Referencias bibliográficas
      Información del producto
      Método de detecciónFluorescente Quimiofluorescente Cromogénico Quimioluminiscencía 125I Fluorescente
      Tinciones compatibles
      • Todos los tintes fluorescentes
      • Azul brillante de Coomassie
      • Negro amido
      • Rojo Ponceau-S
      • CPTS
      • Rubí Sypro®
      Código del filtroIPFL
      Color del filtroBlanco
      Quality LevelMQ400
      Aplicaciones
      Aplicación Transferencia pulg.Southern pulg., transferencia en pocillos ( pulg.dot/slot pulg.)
      Aplicaciones clave
      • Western Blotting
      • Dot Blot
      Información biológica
      Adsorción de BSA205 µg/cm²
      Adsorción de IgG de cabra300 µg/cm²
      Adsorción de insulina155 µg/cm²
      HumectabilidadHidrófoba
      Información fisicoquímica
      Tamaño de poro0.45 µm
      Dimensiones
      Superficie del filtroLisa
      Dimensiones del filtro27 cm x 3.75 m
      Información de materiales
      Química
      • PVDF hidrófobo
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Información de almacenamiento y transporte
      Información sobre embalaje
      Cantitad1
      Información de transporte
      Información complementaria
      Especificaciones
      Global Trade Item Number
      Número de referencia GTIN
      IPFL00010 04053252669439

      Documentation

      Membrana Immobilon-FL, PVDF; 0,45 µm, rollo de 26,5 cm x 3,75 m Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Folleto

      Cargo
      SNAP i.d. 2.0 System Brochure
      Western Blotting Tools

      Licencias necesarias e Información técnica

      Cargo
      Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
      Protein Blotting Handbook: 6th Edition

      Póster

      Cargo
      Low Background Membrane for Fluorescent Protein Detection in Western Blotting

      Manuales del usuario

      Cargo
      Immobilon® Block Noise Cancelling Reagents
      Immobilon®-FL Transfer Membrane User Guide
      User Guide - Immobilon® FL Transfer Membrane