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  • A novel gellan gel-based microcarrier for anchorage-dependent cell delivery. 18434266

    Competent vehicles are highly sought after as a means to transplant cells for tissue regeneration. In this study, novel hydrogel-based microspherical cell carriers are designed and developed with an FDA-approved natural polysaccharide, gellan gum. The bulk fabrication of these microspheres is performed via a water-in-oil (W/O) emulsion process followed by a series of redox (oxidation-reduction) crosslinking treatments; this enables the microspherical dimensions to be precisely manipulated in terms of injectability, and simultaneously ensures the structural stability. To acquire adhesion affinity with anchorage-dependent cells (ADCs), a covalent coating of gelatin is further applied on the microspherical surfaces. The final product is constructed as a variety of gelatin-grafted-gellan microspherical cell carriers, abbreviated as TriG microcarriers. The cell-loading tests are conducted, respectively, with human dermal fibroblasts (HDFs) and human fetal osteoblasts (hFOBs). Morphological observation from optical microscopy and field emission scanning electron microscopy indicates that the HDFs spread well and populate rapidly on surfaces of TriG microcarriers. Immunofluorescent staining reveals the activation of focal adhesion and subsequent organization of F-actin from the attached cell surfaces, which suggests the TriG microspherical substrate is favorable to ADC adhesion and therefore capable of promoting HDF proliferation to achieve confluence by turning over three times within 10 days. The hFOBs are also cultivated on the TriG carriers, where ideal viability and clear potentials for osteogenesis are demonstrated by fluorescent Live/Dead screening and specific histobiochemical indications. All these findings suggest that the TriG microcarriers are suitable to provide open platforms for therapeutic ADC proliferation and differentiation.
    Tipo de documento:
    Referencia
    Referencia del producto:
    FAK100
    Nombre del producto:
    Actin Cytoskeleton / Focal Adhesion Staining Kit

  • Matrix metalloproteinase-9 delays wound healing in a murine wound model. 20004432

    BACKGROUND: Metalloproteinase-9 (MMP-9) is a type IV collagenase found at elevated levels in chronic wounds. As wounds heal, MMP-9 diminishes. In this study, we investigated whether MMP-9 directly contributes to chronic wound pathogenesis. METHODS: Recombinant proMMP-9 was prepared using immortalized keratinocytes transduced by a lentivirus. ProMMP-9 was purified from cell culture media and activated using 4-aminophenylmercuric acetate. Active MMP-9 was then suspended in xanthan gum to a concentration paralleling that found in human chronic wounds. Two parallel 6-mm punch biopsies were made on the backs of C57BL mice. Wounds were treated daily with MMP-9 or vehicle. Wound areas were measured and tissues examined by densitometry, real-time RT-PCR, histology, and immunohistochemistry at days 7, 10, and 12. RESULTS: Exogenous MMP-9, at the level found within chronic wounds, delayed wound healing in this animal model. By 7 days, wounds in the MMP-9-injected group were 12% larger than control wounds (P = .008). By day 12, wounds in the MMP-9-injected group were 25% larger than those of the control group (P = .03). Histologic examination shows that high levels of active MMP-9-impaired epithelial migrating tongues (P = .0008). Moreover, consistent with elevated MMP-9, the collagen IV in the leading edge of the epithelial tongue was diminished. CONCLUSION: MMP-9 appears to directly delay wound healing. Our data suggests that this may occur through interference with re-epithelialization. We propose that MMP-9 interferes with the basement membrane protein structure, which in turn impedes keratinocyte migration, attachment, and the reestablishment of the epidermis.
    Tipo de documento:
    Referencia
    Referencia del producto:
    AB19016
    Nombre del producto:
    Anti-MMP-9 Antibody, Catalytic domain

  • Water in Chewing gum

    Tipo de documento:
    Aplicación
    Referencia del producto:
    Múltiplo
    Nombre del producto:
    Múltiplo

  • Nobiletin Ameliorates the Deficits in Hippocampal BDNF, TrkB, and Synapsin I Induced by Chronic Unpredictable Mild Stress. 23573124

    Background. Our previous study has demonstrated that nobiletin could reverse the behavioral alterations in stressed mice. However, the relation of its antidepressant-like action with neurotrophic molecular expression remains unknown. This study aimed to explore the antidepressant-like mechanism of nobiletin related to the neurotrophic system in rats exposed to chronic unpredictable mild stress (CUMS). Methods. Depressive-like anhedonia (assessed by sucrose preference) and serum corticosterone secretion were evaluated in the CUMS, followed by brain-derived neurotrophic factor (BDNF), its tropomyosin-related kinase receptor B (TrkB), and the downstream target synapsin I expressions in the hippocampus. Results. Anhedonia, which occurred within week 2, was rapidly ameliorated by nobiletin. While fluoxetine needed additional 2 weeks to improve the anhedonia. In addition, nobiletin administration for 5 weeks significantly ameliorated CUMS-induced increase in serum corticosterone levels. Furthermore, we also found that CUMS-induced deficits of hippocampal BDNF, TrkB, and synapsin I were ameliorated by nobiletin. Conclusions. Taken together, these findings suggest that nobiletin produces rapidly acting antidepressant-like responses in the CUMS and imply that BDNF-TrkB pathway may play an important role in the antidepressant-like effect of nobiletin.
    Tipo de documento:
    Referencia
    Referencia del producto:
    AB1543
    Nombre del producto:
    Anti-Synapsin I Antibody

  • Water in Wine gums

    Tipo de documento:
    Aplicación
    Referencia del producto:
    Múltiplo
    Nombre del producto:
    Múltiplo

  • Double edged effect of gum-resin of ferula assa-foetida on lifespan of neurons. 24250948

    Based on knowledge from traditional herbal medicine, Ferula assa-foetida (asafoetida) has several therapeutic applications but there is less knowledge about its effect on neurons.In order to evaluate neuronal differentiation, neuronal like cells were stained against neuronal specific markers β-Tubulin III and MAP2. After establishment of neuronal differentiation in cultured cells, aqueous extract of gum-resin of asafoetida were applied on culture medium of neurons with different concentrations then survival rate of neurons were evaluated by cell counting and methyl tetrazolium bromide (MTT) tests.The results showed that asafoetida gum resin particularly with 0.01 and 1 µg/ml concentrations could improve survival rate of neurons, while10 µgr/ml treated group was toxic.RESULTS of this study indicated that gum resin of asafoetida in low doses has neuroprotective effect on neurons and improves survival rate of them, however in higher concentrations it is toxic for neurons.
    Tipo de documento:
    Referencia
    Referencia del producto:
    AP124F
    Nombre del producto:
    Goat Anti-Mouse IgG Antibody, (H+L) FITC Conjugated

  • Double edged effect of gum-resin of ferula assa-foetida on lifespan of neurons. 24250950

    Based on knowledge from traditional herbal medicine, Ferula assa-foetida (asafoetida) has several therapeutic applications but there is less knowledge about its effect on neurons.In order to evaluate neuronal differentiation, neuronal like cells were stained against neuronal specific markers β-Tubulin III and MAP2. After establishment of neuronal differentiation in cultured cells, aqueous extract of gum-resin of asafoetida were applied on culture medium of neurons with different concentrations then survival rate of neurons were evaluated by cell counting and methyl tetrazolium bromide (MTT) tests.The results showed that asafoetida gum resin particularly with 0.01 and 1 µg/ml concentrations could improve survival rate of neurons, while10 µgr/ml treated group was toxic.RESULTS of this study indicated that gum resin of asafoetida in low doses has neuroprotective effect on neurons and improves survival rate of them, however in higher concentrations it is toxic for neurons.
    Tipo de documento:
    Referencia
    Referencia del producto:
    AP124F
    Nombre del producto:
    Goat Anti-Mouse IgG Antibody, (H+L) FITC Conjugated

  • DNA double-strand breaks and homology search: inferences from a species with incomplete pairing and synapsis. 15976453

    The relationship between meiotic recombination events and different patterns of pairing and synapsis has been analysed in prophase I spermatocytes of the grasshopper Stethophyma grossum, which exhibit very unusual meiotic characteristics, namely (1) the three shortest bivalents achieve full synapsis and do not show chiasma localisation; (2) the remaining eight bivalents show restricted synapsis and proximal chiasma localisation, and (3) the X chromosome remains unsynapsed. We have studied by means of immunofluorescence the localisation of the phosphorylated histone H2AX (gamma-H2AX), which marks the sites of double-strand breaks; the SMC3 cohesin subunit, which is thought to have a close relationship to the development of the axial element (a synaptonemal complex component); and the recombinase RAD51. We observed a marked nuclear polarization of both the maturation of SMC3 cohesin axis and the ulterior appearance of gamma-H2AX and RAD51 foci, these being exclusively restricted to those chromosomal regions that first form cohesin axis stretches. This polarised distribution of recombination events is maintained throughout prophase I over those autosomal regions that are undergoing, or about to undergo, synapsis. We propose that the restricted distribution of recombination events along the chromosomal axes in the spermatocytes is responsible for the incomplete presynaptic homologous alignment and, hence, for the partial synaptonemal complex formation displayed by most bivalents.
    Tipo de documento:
    Referencia
    Referencia del producto:
    Múltiplo
    Nombre del producto:
    Múltiplo