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MABN2640-25UG Anti-BACE1 Antibody, clone 3D5

Anti-BACE1, clone 3D5, Cat. No. MABN2640, is a mouse monoclonal antibody that detects β-Secretase 1 (BACE1) and is tested for use in or use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABN2640-25UG
25 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberMABN2640-25UG
DescriptionAnti-BACE1 Antibody, clone 3D5
Alternate Names
  • Beta-secretase 1
  • EC:3.4.23.46
  • Aspartyl protease 2
  • ASP2
  • Asp 2
  • Beta-site amyloid precursor protein cleaving enzyme 1
  • Beta-site APP cleaving enzyme 1
  • Memapsin-2
  • Membrane-associated aspartic protease 2
Background InformationBeta-secretase 1 (UniProt: P56817; also known as EC:3.4.23.46, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2) is encoded by the BABACE, KIAA1149) gene (Gene ID: 23621) in human. BACE1 is a single-pass type I membrane, monomeric, glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 22-457), a transmembrane domain (aa 458-478), and a cytoplasmic domain (aa 479-501). It also contains a DXXLL motif (aa 496-500) that is required for its proper endocytosis and retrograde transport to the trans-Golgi network, as well as for regulation of lysosomal degradation. BACE1 displays broad endopeptidase specificity and is a rate limiting enzyme that is responsible for the proteolytic processing of amyloid precursor protein (APP). It cleaves at the N-terminus of the Aβ peptide sequence, between Met671 and Asp672 of APP to yield soluble APPβ and C99. The -secretase can then cleave the C99 fragment at either Val711 or Ile713 to produce the shorter Aβ peptide (Aβ1-40) or the longer Aβ peptide (Aβ1-42). Aβ plaques are reported to induce BACE1 activity in surrounding neurons at early stages of Alzheimer s disease before neuronal death occurs. BACE1 undergoes acetylation at multiple lysine residues (126, 275, 279, 285, 299, 300, and 307) that regulates its maturation, transport to plasma membrane, and contributes to its stability. It also undergoes phosphorylation at serine 498, which is required for its interaction with GGA1 and retrograded transport from endosomal compartment to the trans-Golgi network. Six isoforms of BACE1 have been described that are produced by alternative splicing. (Ref.: Sadleir, KR., et al. (2013). Curr Alzheimer Res. 10(8); 785-789; Zhao, J., et all (2007). J. Neurosci. 27(14); :3639 -3649; Constantini, C., et al. (2007). Biochem. J. 407(3); 383-395).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-BACE1, clone 3D5, Cat. No. MABN2640, is a mouse monoclonal antibody that detects β-Secretase 1 (BACE1) and is tested for use in or use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • ELISA
  • Immunofluorescence
  • Immunocytochemistry
Application NotesTested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected BACE1 in NIH/3T3 cell lysate.

Immunohistochemistry Applications: A representative lot detected BACE1 in Immunohistochemistry applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

Immunofluorescence Analysis: A representative lot detected BACE1 in Immunofluorescence applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected BACE1 in HeLa cells.

ELISA Analysis: A representative lot detected BACE1 in ELISA applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49).

Western Blotting Analysis: A representative lot detected BACE1 in Western Blotting applications (Zhao, J., et al. (2007). J Neurosci. 27(14):3639-49; Sadleir, K.R., et al. (2013). Curr Alzheimer Res. 10(8):785--9; Pigoni, M. et al. (2020). EMBO J. 39(15):e103457).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 415 amino acids from the internal region of human β-secretase 1 (BACE1).
EpitopeInternal
Clone3D5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3D5 is a mouse monoclonal antibody that detects β-secretase 1 (BACE1).
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BACE1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~57 kDa observed; 55.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BACE1 in SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABN2640-25UG 04065270451736